Characteristics of some digestive enzymes in sobaity, Sparidentex hasta

Document Type : Clinical Pathology


Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran


Background: Determination of digestive enzymes activity would provide critical information in the design of appropriate diet. Sobaity, Sparidentex hasta is found in the Persian Gulf and  cultured in countries adjacent to it. OBJECTIVES: This study investigated proteolytic, amylase and lipase activities in the intestine of Sparidentex hasta captured from the  Persian Gulf. METHODS: 60 immature S. hasta (mean weight: 520 ± 50g) were captured during summer and autumn of 2012 from Mussa Creek, North West of the Persian Gulf. After euthanization, fish were dissected and the complete digestive tracts (from stomach to anus) were removed. The intestines were separated for each fish and divided to 3 parts; proximal, mid portion and distal. After homogenization, supernatants were removed and enzymes activities were assayed chemically. RESULTS: The highest activity of protease (395.2 ± 32.6 mU mg-1 protein) was recorded in the proximal portion of the intestine compare to the 2 other parts (p<0.05) but amylase and lipase activities did not show a significant difference in 3 parts of the intestine (p>0.05). CONCLUSIONS: This study showed that the pattern of activity of digestive enzymes in S. hasta is consistent with the overall pattern of digestive enzymes activity in carnivorous fish. These data can be used to design special diets for this species.


Article Title [Persian]

ویژگیهای برخی از آنزیم های گوارشی در ماهی صبیتی Sparidentex hasta

Author [Persian]

  • مهدی جهان تیغ
گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه زابل، زابل، ایران
Abstract [Persian]

زمینه مطالعه:  فعالیت آنزیم‌‌های گوارشی نقش مهمی در جذب مواد مغذی و رشد ماهیان دارد. با این وجود اطلاعات موجود در زمینه فعالیت این آنزیمها در ماهیان دریایی خلیج فارس اندک میباشد و مطالعات جامعی در این زمینه انجام نگرفته است. هدف: این مطالعه فعالیت آنزیمهای پروتئولیتیک، لیپاز و آمیلاز در روده ماهی صبیتی، Sparidentex hasta صید شده در خلیج فارس را مورد بررسی قرار میدهد. روش کار: در این مطالعه برای دست یابی به اطلاعات پایه در زمینه فعالیت این آنزیمها در روده ماهی صبیتی، تعداد 60 ماهی نابالغ با میانگین وزن g‌50±520 از خور موسی صید گردید. بعد از آسان کشی ماهیان، دستگاه گوارش از دهان تا مخرج جدا و روده به 3 قسمت ابتدایی، میانی و انتهایی تفکیک شد. پس از هموژن سازی رودهها، نمونهها سانتریفیوژ و مایع رویی جدا گردید. سپس فعالیت آنزیمها به روش شیمیایی و با استفاده از اسپکتروفتومتر اندازهگیری گردید. نتایج: بیشترین فعالیت لیپاز و پروتئاز در قسمت ابتدایی روده ثبت گردید که در مقایسه با دو قسمت دیگر اختلاف معنیداری نشان داد (05/0>p) اما فعالیت پروتئاز در 3 بخش روده اختلاف معنیداری نشان نداد. نتیجهگیرینهایی: نتایج این مطالعه نشان داد که الگوی فعالیت آنزیمهای گوارشی در این ماهی از الگوی کلی فعالیت آنزیمهای گوارشی در ماهیان گوشتخوار پیروی میکند.

Keywords [Persian]

  • آمیلاز
  • لیپاز
  • پروتئاز
  • Sparidentex hasta
  • خلیج فارس
Bier, M., (1955) Lipases. Methods in Enzymology I. Academic Press, New York. p. 627–642.
Buddington, R.K., Krogdahl, A., Bakke-Mckellep, A.M. (1997) The intestine of carnivorous fish: structure and functions and the relations with diet. Acta Physiol Scand. 161: 67-80.
Chakrabarti, I., Gani, M.A., Chaki, K.K., Sur, R., Misra, K.K. (1995) Digestive enzymes in 11 freshwater teleost fish species in relation to food habit and niche segregation. Comp Biochem Physiol A. 112: 167–177.
Chaudhuri, A., Mukherjee, S., Homechaudhuri, S. (2012) Diet composition and digestive enzymes activity in carnivorous fishes inhabiting mudflats of Indian sundarban estuaries. Turk J Fish Aquat Sci. 12: 265-275.
Correa, C.F., De Aguiar, L.H., Lundstedt, L.M., Moraes, G. (2007). Responses of digestive enzymes of tambaqui (Colossoma macropomum) to dietary cornstarch changes and metabolic inferences. Comp Biochem Physiol A. 147: 857–862.
Cuvier-Peres, A., Kestemont, P. (2002) Development of some digestive enzymes in Eurasian perch larvae Perca fluviatilis. Fish Physiol Biochem. 24: 279–285.
De Almeida, L.C., Lundstedt, L.M., Moraes, G. (2006) Digestive enzyme responses of Tam baqui (Colossoma macropomum) fed on different levels of protein and lipid. Aquac Nutr. 12: 443–450.
Deguara, S., Jauncey, K., Agius, C. (2003) Enzyme activities and pH variations in the digestive tract of gilthead sea bream. J Fish Biol. 62: 1033–1043.
Fountoulaki, E., Alexis, M.N., Nengas, I., Venou, B. (2005) Effect of diet composition on nutrient digestibility and digestive enzyme levels of gilthead sea bream (Sparus aurata L.). Aquacult Res. 36: 1243–1251.
Furne, M., Hidalgo, M.C., Lopez, A., Garcıa-Gallego, M., Morales, A.E., Domezain, A., Domezaine, J., Sanz, A. (2005) Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss, A comparative study. Aquaculture. 250: 391–398.
Hidalgo, M.C., Urea E., Sanz A. (1999) Comparative study of digestive enzymes in fish with different nutritional habits, Proteolytic and amylase activities. Aquaculture. 170: 267–28.
Izvekova, G.I., Solovyev, M.M., Kashinskaya, E.N., Izvekov, E.I. (2013) Variations in the activity of digestive enzymes along the intestine of the burbot Lota lota expressed by different methods. Fish Physiol Biochem. 39: 1181-93.
Ji, H., Sun, H.T., Xiong, D.M. (2012) Studies on activity, distribution, and zymogram of protease, α-amylase, and lipase in the paddlefish Polyodon spathula. Fish Physiol Biochem. 38: 603-613.
Liu, Z.Y., Wang, Z., Xu, S.Y., Xu, L.N. (2008) Partial characterization and activity distribution of proteases along the intestine of grass carp, Ctenopharyngodon idella (Val.). Aquac Nutr. 14: 31–39.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with Folin phenol reagent. J Biol Chem. 193: 265–275.
Lundstedt, L.M., Fernando Bibiano Melo, J., Moraes, G. (2004) Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans (Teleostei: Siluriformes) in response to diet composition. Comp Biochem Physiol B. 137: 331–339.
Metin, K., Akpınar, M.A. (2000) Characterization of lipase in intestine of Oncorhynchus mykiss Walbaum, 1792. Turkish. Turk J Biol. 24: 489-502.
Natalia, Y., Hashim, R., Ali, A., Chong, A. (2004) Characterization of digestive enzymes in a carnivorous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae). Aquaculture. 233: 305–320.
Perez-Casanova, J.C., Murray, H.M., Gallant, J.W., Ross, N.W., Douglas, S.E., Johnson, S.C. (2004) Bile salt-activated lipase expression during larval development in the haddock (Melanogrammus aeglefinus). Aquaculture. 235: 601–617.
Pérez-Jiménez, A., Cardenete, G., Morales, A.E., Garcia-Alcazar, A., Abellan, E., Hidalgo, M.C. (2009) Digestive enzymatic profile of Dentex dentex and response to different dietary formulations. Comp Biochem Physiol. 154: 157-164.
Phillips, A.M.Jr. (1969) Nutrition, digestion and energy utilization. In: Fish Physiology.  Hoar, W.S., Randall, D.J. (eds.). Academic Press. London, UK. p. 391-432.
Ribeiro, L., Zambonino-Infante, J.L., Cahu, C., Dinis, M.T. (1999) Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858. Aquaculture. 179: 465–473.
Robyt, J.F., Whelan, W.J. (1968) The h-amylases. In: Starch and Its Derivates. Radley, J.A. (ed.). Academic Press, London, UK. p. 477– 497.
Sklan, D., Prag, T., Lupatsch, I. (2004) Structure and function of the small intestine of the tilapia Oreochromis niloticus, Oreochromis aureus (Teleostei, Cichlidae). Aquac Res. 35: 350–357.
Thompson, K.R., Muzinic, L.A., Engler, L.S., Webster, C.D. (2005) Evaluation of practical diets containing different protein levels, with or without fish meal, for juvenile Australian red claw crayfish (Cherax quadricarinatus). Aquaculture. 244: 241–249.
Tramati, C., Savona, B., Mazzola, A. (2005) A study of the pattern of digestive enzymes in Diplodus puntazzo (Cetti, 1777) (Osteichthyes, Sparidae): evidence for the definition of nutritional protocols. Aquac Int. 13: 89–95.
Ugolev, A.M., Iezuitova, N.N. (1985) Membrane digestion. Structural and functional organization. Usp Fiziol Nauk. Adv Physiol Sci. 16: 3–34.
Walter, H.E. (1984) Proteinases: methods with hemoglobin, casein and azocoll as substrates. In: Methods of Enzymatic Analysis. Bergmeyer, H.U. (ed.). vol. V. Verlag Chemie, Weinheim, Germany. p. 270- 277.