Effect of dietary zinc oxide and phytase on the plasma metabolites and enzyme activities in aged broiler breeder hens

Document Type : Nutrition - Hygiene


1 Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran


Background: It has been shown that zinc has an effect on physiological responses in animals and birds. On the other hand, dietary phytase in poultry results in increased availability of zinc. OBJECTIVES: This study was conducted to investigate the effects of zinc oxide (ZnO) and Escherichia coli-derived 6-phytase supplemented diets on the plasma metabolites and enzyme activities of broiler breeder hens from 60 to 72 weeks of age. METHODS: A total of 128 breeder hens were randomly assigned to eight dietary treatments, with four replicates of four hens each. Blood concentration of Zn, Ca, P, total protein, cholesterol, triglyceride (TG) and high density lipoprotein (HDL), and plasma activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were measured. RESULTS: Results showed that supplementary ZnO increased plasma Zn, Ca, P, HDL, and total protein (p<0.01) concentrations, as well as enzyme activities of LDH, ALT and AST (p<0.01). Also, a ZnO-supplemented diet resulted in a decrease in plasma cholesterol and TG (p<0.01) levels. Adding phytase to the diet increased plasma (P) and HDL contents (p<0.01). The interactive effect of phytase × ZnO × period on the plasma levels of Zn, P, total protein, HDL, total cholesterol, and the enzymatic activity of LDH, ALT and ALP was significant. CONCLUSIONS: It is concluded that supplementary ZnO and phytase may improve metabolism and enzymatic activity of aged broiler breeder hens.


Article Title [Persian]

اثر اکسید روی و فیتاز جیره بر متابولیتهای پلاسما و فعالیتهای آنزیمی در مرغهای مادر مسن

Authors [Persian]

  • حسین شریده 1
  • مهدی ژندی 1
  • مجتبی زاغری 1
  • امیر اخلاقی 2
1 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران
Abstract [Persian]

زمینه مطالعه:  اثر روی بر پاسخهای فیزولوژیک در حیوانات و پرندگان نشان داده شده است. از طرف دیگر، فیتاز جیره در طیور منجر به افزایش دسترسی روی میشود. هدف: این مطالعه برای بررسی اثرات جیرههای مکمل شده با اکسید روی و 6-فیتاز مشتق شده از E. Coli بر متابولیتها و فعالیتهای آنزیمی پلاسما در مرغهای مادر گوشتی مسن از سن 60 تا 72 هفتگی انجام شد. روش‌‌کار: تعداد 128 مرغ مادر به صورت تصادفی به هشت تیمار تغذیهای با چهار تکرار و چهار مرغ در هر تکرار اختصاص پیدا کردند. غلظت خونی روی، کلسیم، پروتئین کل، کلسترول، تریگلیسرید و HDL و فعالیت پلاسمایی AST، ALT، ALP و LDH اندازهگیری شدند. نتایج: نتایج نشان داد که مکمل روی با افزایش پلاسمایی غلظتهای روی، کلسیم، فسفر، HDL و پروتئین کل (01/0<p)، به علاوه با افزایش فعالیت آنزیمهای LDH، ALT و AST (01/0< p) همراه بود. همچنین، جیره مکمل شده با روی منجر به کاهشی در سطوح کلسترول و تریگلیسرید شد (01/0<p). افزودن فیتاز به جیره مقدار فسفر و HDL را افزایش داد (01/0<p). اثر متقابل فیتاز، اکسید روی و دوره بر سطوح پلاسمایی روی، فسفر، پروتئین کل وHDL کلسترول کل و فعالیت آنزیمی LDH، ALT و ALP معنیدار بود. نتیجهگیرینهایی: نتیجهگیری میشود که مکمل اکسید روی و فیتاز ممکن است متابولیسم و فعالیت آنزیمی در مرغهای مادر گوشتی مسن را بهبود دهد.

Keywords [Persian]

  • آنزیم
  • مرغ
  • متابولیت
  • فیتاز
  • روی
Aksu, D., Aksu, T., Ozsoy, B. (2010) The effects of lower supplementation levels of organically complexed minerals (zinc, copper and manganese) versus inorganic forms on haematological and biochemical parameters in broiler. Kafkas Univ Vet Fak Derg. 16: 553-559.
Armstrong, J.D., Benoit, A.M. (1996) Paracrine, autocrine, and endocrine factors that Mediate the influence of nutrition on reproduction in cattle and swine: an in vivo, IGF-I perspective. J Anim Sci. 74: 18-35.
Broz, J., Oldale, P., Perrin‐Voltz, A.H., Rychen, G., Schulze, J., Nunes, C.S. (1994) Effects of supplemental phytase on performance and phosphorus utilisation in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. Br Poult Sci. 35: 273-280.
Chen, S., McMurtry, J., Walzem, R. (2006) Overfeeding-induced ovarian dysfunction in broiler breeder hens is associated with lipotoxicity. Poult Sci. 85: 70-81.
Cheryan, M. (1980) Phytic acid interactions in food systems. Crit Rev Food Sci. 13: 297-335.
Eder, K., Kirchgessner, M. (1995) Zinc deficiency and activities of lipogenic and glycolytic enzymes in liver of rats fed coconut oil or linseed oil. Lipids. 30: 63-69.
Eltohamy, M.M., Younis, M. (1991) Response of testes, epididymis, and seminal vesicle of rabbits to zinc deficiency. Arch Exp Vet Med. 45: 155-160.
Feng, J., Ma, W.Q., Niu, H.H., Wu, X.M., Wang, Y. (2010) Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biol Trace Elemt Res. 133: 203-211.
Jondreville, C., Lescoat, P., Magnin, M., Feuerstein, D., Gruenberg, B., Nys, Y. (2007) Sparing effect of microbial phytase on zinc supplementation in maize-soya-bean meal diets for chickens. Animal. 1: 804-811.
Herzig, I., Navratilova, M., Totusek, J., Suchy, P., Vecerek, V., Blahova, J., Zraly, Z. (2009) The effect of humic acid on zinc accumulation in chicken broiler tissues. Czech J Anim Sci. 54: 121-127.
Lim, H.S., Namkung, H., Paik, I.K. (2003) Effects of phytase supplementation on the performance, egg quality, and phosphorous excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorous. Poult Sci. 82: 92-99
Liu, B.L., Rafiq, A., Tzeng, Y.M., Rob, A. (1998) The Induction and Characterization of Phytase and Beyond. Enzyme Microb Technol. 22: 415-424.
Maenz, D., Classen, H. (1998) Phytase activity in the small intestinal brush border membrane of the chicken. Poult Sci. 77: 557-563.
Mohanna, C., Nys, Y. (1999) Changes in zinc and manganese availability in broiler chicks induced by vegetal and microbial phytases. Anim Feed Sci Technol. 77: 241-253.
Park, S.Y., Birkhold, S.G., Kubena, L.F., Nisbet, D.J., Ricke, S.C. (2004) Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction. Biol Trace Elem Res. 101: 147-163.
Paul, A., Calleja, L., Joven, J., Vilella, E., Ferre, N., Camps, J., Girona, J., Osada, J. (2001) Supplementation with vitamin E and/or zinc does not attenuate atherosclerosis in apolipoprotein E-deficient mice fed a high-fat, high-cholesterol diet. Int J Vitam Nutr Res. 71: 45-52.
Prasad, A.S. (1996) Zinc deficiency in women, infants and children. J Am Coll Nutr. 15: 113-120.
Roberson, K.D., Edwards, H.M. Jr. (1994) Effects of 1,25-dihydroxycholecalciferol and phytase on zinc utilization in broiler chicks. Poult Sci. 73: 1312-1326.
Rupic, V., Ivandija, L., Luterotti, S., Dominis-Kramaric, M. (1997) Influence of inorganic and organic dietary zinc on its concentration in blood serum, bones and hair, and catalytical activity of selected serum enzymes in pigs. Acta Vet Brno. 66: 75-85.
Viveros, A., Brenes, A., Arija, I., Centeno, C. (2002) Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poult Sci. 81: 1172-1183.
Weaver, C.M., Kannan, S. (2002) Phytate and mineral bioavailability. In: Food Phytates. Reddy, N.R., Sathe. S.K. (eds.). (1st ed.) CRC Press, Boca Raton, FL, USA. p. 211–224.
Yousef, M.I., El-Hendy, H.A., El-Demerdash, F.M., Elagamy, E.I. (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology. 175: 223-234.
Wilson, H.(1997) Effects of maternal nutrition on hatchability. Poult Sci. 76: 134-143.
Zaghari, M., Sedaghat, V., Shivazad, M. (2013) Effect of vitamin E on reproductive performance of heavy broiler breeder hens. J Appl Poult Res. 22: 808-813.
Zhang, Y.H., Cheng, Y.Y., Hong, Y., Wang, D.L., Li, S.T. (2003) Effects of zinc deficiency on bone mineralization and its mechanism in rats. Zhonghua Yu Fang Yi Xue Za Zhi. 37: 121-124.
Zyla, K., Dulinski, R., Pierzchalska, M., Grabacka, M., Jozefiak, D., Swiatkiewicz, S. (2013) Phytases and myo-inositol modulate performance, bone mineralization and alter lipid fractions in the serum of broilers. J Anim Feed Sci. 22: 56-62.