A study on the presence of some potential virulence genes and quinolone resistance in avian pathogenic Escherichia coli isolated from chickens in Northeast of Iran

Document Type : Infectious agents- Diseases


Department of Pathobiology, School of Veterinary Medicine, Ferdowsi Unversity of Mashhad, Mashhad, Iran


Background: Avian pathogenic Escherichia coli (APEC), which is the causative agent of colibacillosis, harbors several putative virulence genes. An important trait of APEC for both poultry and public health is antibiotic resistance. Objectives: In the present study, some potential virulence genes of APECs isolated from Northeast of Iran and their resistance to the quinolones antibiotics were studied. Methods: The conventional polymerase chain reaction (PCR) was used to determine the presence of four virulence genes, including iss, cvi, iuc, and tsh, in 52 isolates of E. coli from avian colibacillosis and 11 isolates from feces of apparently healthy chicken. Disk diffusion method was used to also determine the resistance of all the isolates against nalidixic acid, norofloxacin, ciprofloxacin, and enrofloxacin. Results: The presence of iss and tsh virulence genes in isolates from diseased chickens was significantly higher than isolates from healthy chickens. There was no significant difference between APEC and fecal E. coli when it comes to quinolone resistance. However, cvi and iuc genes were significantly higher in susceptible isolates of E. coli from healthy chickens. Conclusions: iss and tsh genes are more prevalent in APEC isolates than in fecal isolates. There is no association between lack of virulence and resistance to quinolones in E. coli isolates from diseased chickens.


Article Title [Persian]

بررسی وجود تعدادی از ژنهای بالقوه حدتزا و مقاومت به کینولونها در سویههای بیماریزای اشریشیا کلی جدا شده از طیور در شمال شرق ایران

Authors [Persian]

  • مهرناز راد
  • مژگان کوشان
گروه پاتوبیولوژی دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
Abstract [Persian]

زمینه مطالعه:  اشریشیا کلی بیماریزای طیور (APEC)، عامل مسبب کلی باسیلوز بوده که دارای بسیاری از ژنهای بالقوه حدتزا میباشد. هدف: در این مطالعه تعدادی از ژنهای بالقوه حدتزا در سویههای APEC و مقاومت آنها به کینولونها مورد بررسی قرار گرفتند. روش کار: وجود چهار ژن حدت شامل iss، cvi،iuc و tsh در 52 جدایه اشریشیا کلی از کلی باسیلوز طیور و 11 جدایه از مدفوع طیور به ظاهر سالم با روش قراردادی PCR مورد بررسی قرار گرفت. مقاومت تمام جدایهها در برابرنالیدیکسیک اسید، نوروفلوکساسین، سیپروفلوکساسین و انروفلوکساسین با روش انتشار از دیسک ارزیابی گردید. نتایج: وجود ژنهای iss و tshدر جدایههای طیور بیمار به طور معنیداری نسبت به جدایههای طیور سالم بیشتربود. تفاوت معنیداری بین سویههای APEC و اشریشیاکلی مدفوعی در مقاومت به کینولونها وجود نداشت. ولی، ژنهای cvi و iuc به طور معنیداری در جدایههای حساس به کینولون در طیورسالم، بیشتر بود. نتیجهگیرینهایی:  ژنهای tsh و iss در بیماریزایی سویههای اشریشیا کلی اهمیت دارند. ارتباطی بین عدم وجود ژن حدت  و مقاومت در برابر کینولونها در جدایههای طیور بیمار مشاهده نشد. 

Keywords [Persian]

  • اشریشیا کلی طیور
  • کلی باسیلوز
  • مقاومت به کینولون
  • عوامل حدت
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