Effects of feed restriction and dietary fat type on mRNA expression of liver fatty acid-binding protein (L-FABP) in broilers

Document Type : Nutrition - Hygiene

Authors

1 Depatment of Animal Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

2 Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran

3 Agricultural Biotechnology Research Institute of north of Iran, Rasht. Iran

4 Depatment of Animal Science, Bu-Ali Sina University, Hamedan, Iran.

Abstract

Background: Liver fatty acid-binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance the uptake of fatty acids into the cell by increasing their concentration due to decreasing concentration of unbound fatty acids inside the cell. Objectives: The aim of this study was to evaluate the effects of dietary unsaturated to saturated fatty acid ratio and feed restriction on L-FABP mRNA expression. Methods: A total of 720, 10-day old male Ross 308 broiler chicks were fed diets with unsaturated to saturated fatty acid ratio (U/S) of 2, 3.5, 5, or 6.5 as ad libitum or skip-a-day feeding schedule (during 18–28 days of age). Relative expression of L-FABP mRNA in hepatocytes of broilers was determined using quantitative reverse transcription real-time polymerase chain reaction. Results: Our results show that feed restriction induced the expression of L-FABP gene in the liver of broilers. Moreover, L-FABP gene expression was increased by dietary U/S ratio of 6.5. There was no interaction between dietary U/S and feed restriction on the L-FABP gene expression. Conclusions: Results indicate that birds have a mechanism for regulation of fatty acid transfer under different nutritional conditions.

Keywords

References

Bass, N.M. (1988) The cellular fatty acid binding proteins: aspects of structure, regulation and function. Int Rev Cytol. 3: 143–184.
Berry, S.A., Yoon, J.B., List, J., Seelig, S. (1993) Hepatic fatty acid-binding protein mRNA is regulated by growth hormone. J Am Coll Nutr. 12: 638–642.
Carlsson, L., Nilsson, I., Oscarsson, J. (1998) Hormonal regulation of liver fatty acid binding protein in vivo and in vitro: effects of growth hormone and insulin. Endocrinology. 139: 2699–2709.
Coe, N.R., Bernlohr, D.A. (1998) Physiological properties and functions of intracellular fatty acid-binding proteins. Biochem Biophys. Acta- Proteins and Proteomics. 1391: 287–306.
Di Pietro, S.M., Veerkamp, J.H., Santome, J.A. (1999) Isolation, amino acid sequence determination and binding properties of two fatty-acid-binding proteins from axolotl (Ambistoma mexicanum) liver–evolutionary relationship. Eur J Biochem. 259: 127–134.
Duplus, E., Glorian, M., Foresti, C. (2000) Fatty acid regulation of gene transcription. J Biol Biochem. 275: 30749–30752.
Freeman, B.M. (1984) Physiology and Biochemistry of the Domestic Fowl, Vol 5. Academic Press, London, UK.
Gao, G.L., Na, W., Wang, Y.X., Zhang, H.F., Li, H., Wang, Q.G. (2015) Role of a liver fatty acid-binding protein gene in lipid metabolism in chicken hepatocytes. Genet Mol Res. 14: 4847-4857.
Glatz, J.F., Vander Vusse, G.J. (1996) Cellular fatty acid-binding proteins: their function and physiological significance. Prog Lipid Res. 35: 243–282.
Haunerland, N.H., Spener, F. (2004) Fatty acid-binding proteins–insights from genetic manipulations. Prog Lipid Res. 43: 328–349.
Hulan, H.W., Proudfoot, F.G., Nash, D.M. (1984) The effects of different dietary fat sources on general performance and carcass fatty acid composition broiler chickens. Poultry Sci. 63: 324-332.
Huang, J., Yang, D., Gao, S., Wang, T. (2008) Effects of soy-lecithin on lipid metabolism and hepatic expression of lipogenic genes in broiler chickens. Livest Sci. 118: 53–60.
Livak, K.J., Schmittengen, T.D. (2001) Methods. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods. 25: 402–408.
Murai, A., Furuse, M., Kitaguchi, K., Kusumoto, K., Nakanishi, Y., Kobayashi, M., Horio, F. (2009) Characterization of critical factors influencing gene expression of two types of fatty acid-binding proteins (L-FABP and Lb-FABP) in the liver of birds. Comp Biochem Physiol, Part A. 154: 216–223.
Newberry, E.P., Xie, Y., Kennedy, S., Han, X., Buhman, K.K., Luo, J., Gross, R.W., Davidson, N.O. (2003) Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene. J Biol Biochem. 278: 51664–51672.
Ockner, R.K., Manning, J.A. (1974) Fatty acid-binding protein in small intestine: Identification, isolation, and evidence for its role in cellular fatty acid transport. J Clin Invest. 54: 326-338.
Prows, D.R., Murphy, E.J., Schroeder, F. (1995) Intestinal and liver fatty acid binding proteins differentially affect fatty acid uptake and esterification in L-cells. Lipids. 30: 907–910.
Richards, M.P., Poch, S.M., Coon, C.N., Rosebrough, R.W., Ashwell, C.M., McMurtry, J.P. (2003) Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. J Nutr. 133: 707–715.
Spann, N.J., Kang, S., Li, A.C., Chen, A.Z., Newberry, E.P., Davidson, N.O., Hui, S.T., Davis, R.A. (2006) Coordinate transcriptional repression of liver fatty acid-binding protein and microsomal triglyceride transfer protein blocks hepatic very low density lipoprotein secretion without hepatosteatosis. J Biol Chem. 281: 33066–33077.
Storch, J., Thumser, A.E.A. (2000) The fatty acid transport functions of fatty acid-binding proteins. Biochem Biophys Acta-Proteins and Proteomics. 1486: 28-44.
Storch, J., Corsico, B. (2008) The emerging functions and mechanisms of mammalian fatty acid-binding proteins. Ann Rev Nut. 28: 73–95.
Veerkamp, J.H. (1995) Fatty acid transport and fatty acid-binding proteins. P Nutr Soc. 54: 23–37.
Weisiger, R.A. (1996) Cytoplasmic transport of lipids: role of binding proteins. Comp Biochem Physiol. 115: 319-331.
Wolfrum, C., Borrmann, C.M., Börchers, T., Spener, F. (2001) Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors α- and γ- mediated gene expression via liver fatty acid binding protein: a signaling path to the nucleus. P Natl A Sci. USA. 98: 2323–2328.
Yuan, Y., Zhang, B., Guo, Y. (2012) Poultry fat decreased fatty acid transporter protein mRNA expression and affected fatty acid composition in chickens. J Anim Sci Biotechnol. 3: 17.
Yu, M.W., Robinson, F.E. (1992) The application of short-term feed restriction to broilers chickens production: a review. J Appl Poult Res. 1: 147-153.
Yu, Y., Wang, D., Sun, D.X., Xu, G.Y., Li, J.Y., Zhang, Y. (2011) Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens. Yi Chuan. 33: 763-767.
Zhang, Q., Shi, H., Liu, W., Wang, Y., Wang, Q., Li, H. (2013) Differential expression of L-FABP and L-BABP between fat and lean chickens. Genet Mol Res. 7: 4192-4206.
Iranian Journal of Veterinary Medicine
Volume 9, Issue 4 - Serial Number 4
January 2016
Pages 279-286

Files

Share

How to cite

Statistics