Effects of feed restriction and dietary fat type on mRNA expression of liver fatty acid-binding protein (L-FABP) in broilers

Document Type: Nutrition - Hygiene

Authors

1 Depatment of Animal Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

2 Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran

3 Agricultural Biotechnology Research Institute of north of Iran, Rasht. Iran

4 Depatment of Animal Science, Bu-Ali Sina University, Hamedan, Iran.

Abstract

Background: Liver fatty acid-binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance the uptake of fatty acids into the cell by increasing their concentration due to decreasing concentration of unbound fatty acids inside the cell. Objectives: The aim of this study was to evaluate the effects of dietary unsaturated to saturated fatty acid ratio and feed restriction on L-FABP mRNA expression. Methods: A total of 720, 10-day old male Ross 308 broiler chicks were fed diets with unsaturated to saturated fatty acid ratio (U/S) of 2, 3.5, 5, or 6.5 as ad libitum or skip-a-day feeding schedule (during 18–28 days of age). Relative expression of L-FABP mRNA in hepatocytes of broilers was determined using quantitative reverse transcription real-time polymerase chain reaction. Results: Our results show that feed restriction induced the expression of L-FABP gene in the liver of broilers. Moreover, L-FABP gene expression was increased by dietary U/S ratio of 6.5. There was no interaction between dietary U/S and feed restriction on the L-FABP gene expression. Conclusions: Results indicate that birds have a mechanism for regulation of fatty acid transfer under different nutritional conditions.

Keywords


Article Title [Persian]

اثر محدودیت غذایی و نوع چربی جیره بر بیان mRNA پروتئین متصل شونده به اسید چرب در کبد جوجههای گوشتی

Authors [Persian]

  • بهمن نویدشاد 1
  • قاسم حسینی سالکده 2
  • مریم رویان 3
  • مصطفی ملکی 4
1 گروه علوم دامی، دانشگاه محقق اردبیلی، اردبیل، ایران
2 پژوهشکده بیوتکنولوژی کشاورزی ایران
3 پژوهشکده بیوتکنولوژی کشاورزی شمال کشور،
4 گروه علوم دامی، دانشگاه بوعلی سینا ، همدان، ایران
Abstract [Persian]

زمینه مطالعه:  پروتئین متصل شونده به اسید چرب مخصوص کبد (L-FABP) جایگاه اصلی اتصال سیتوزولی اسیدهای چرب زنجیر بلند در سلول‌های کبدی است. FABPs جذب اسیدهای چرب به داخل سلول را افزایش می‌دهند و این عمل را از طریق افزایش شیب غلظتی آنها به دلیل به حداقل رساندن میزان اسید چرب غیر متصل در سلول انجام می‌دهند. هدف: هدف از این تحقیق بررسی اثرات نسبت اسیدهای چرب غیر اشباع به اشباع جیره و محدودیت غذایی بر بیان mRNA پروتئین متصل شونده به اسید چرب مخصوص کبد بود. روش کار: تعداد 720 قطعه جوجه گوشتی نر 10 روزه از سویه رأس 308 با جیره‌های بانسبت‌های اسیدهای چرب غیر اشباع به اشباع (U/S) 2، 5/3، 5 یا 5/6 به‌صورت آزاد یا یک‌روز در میان تغذیه گردیدند (طی سن 28-18 روزگی). بیان نسبی mRNA  مربوط به L-FABP در سلول‌های کبدی جوجه‌ها با استفاده از نسخه برداری معکوس طی روش real time PCR تعیین گردید. نتایج: نتایج به روشنی نشان دادند که محدودیت غذایی باعث القاء بیان ژن در کبد جوجه‌های گوشتی گردید. بیان ژن L-FABP توسط نسبت 5/6 اسیدهای چرب غیر اشباع به اشباع جیره افزایش یافت. اثر متقابلی بین U/S جیره و محدودیت غذایی بر بیان ژن L-FABP مشاهده نگردید. نتیجهگیرینهایی: این مشاهدات پیشنهاد می‌نمایند که پرندگان دارای سازوکاری برای تنظیم انتقال اسیدهای چرب تحت شرایط مختلف تغذیه‌ای هستند.

Keywords [Persian]

  • جوجه­ های گوشتی
  • نسبت اسیدهای چرب غیر اشباع و اشباع جیره
  • محدودیت غذایی
  • پروتئین متصل شونده به اسید چرب
  • ریل تایم پی سی آر
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