The effect of pure phenol on sperm parameters and fertility rate in male mice

Document Type : Pharmacology


1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Anatomy, Tehran Medical Branch, Islamic Azad University, Tehran, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

4 Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


Background: Phenol is an estrogenic and toxic compound and people are widely exposed to it, in different ways.  OBJECTIVES: The aim of this study was to determine  the  negative effects of phenol on the fertility of male mice, by investigating sperm parameters including viability, motility, fertility rates and daily sperm production (DSP). METHODS: A total of 106 adult NIH mice were divided into four groups, one control and three experimental groups (n=13). The treatment groups were given daily dose of phenol for 35 days through gavage method (30, 75 and 100 mg/kg) while the control group received only normal saline. At day 36, six mice were sacrificed from each group. Gonadosomatic index (GSI), viability, motility and daily sperm production were determined carefully. The remaining 7 mice from each group were used to mate with 2 female mice. On GD10, the female mice were sacrificed and the fertility was verified. RESULTS: Daily sperm production in treatment groups decreased significantly compared to the control group (p≤0.05). Body weight, sperm motility, viability and fertility percentage were significantly decreased in 75 and 100 mg/kg groups in comparison with the control group (p≤0.05). While the testes showed no significant changes in weight in any groups. Gonadosomatic index in the treatment groups compared to the control group, was significantly decreased (p≤0.05). CONCLUSIONS: Pure phenol could reduce fertility rate through decreasing motility, daily sperm production and sperm viability.


Article Title [Persian]

اثر فنل خالص بر پارامترهای اسپرم و میزان باروری در موش سوری نر

Authors [Persian]

  • زهرا طوطیان 1
  • سیمین فاضلی پور 2
  • نادر گودرزی 3
  • حسینعلی عرب 4
1 گروه علوم پایه، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
2 گروه آناتومی، دانشکده پزشکی ، دانشگاه آزاد اسلامی واحد تهران ، تهران-ایران
3 گروه علوم پایه، دانشکده دامپزشکی دانشگاه رازی، کرمانشاه- ایران
4 4) گروه فارماکولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران- ایران
Abstract [Persian]

زمینه مطالعه:  فنل ترکیبی استروژنیک و سمی است که اغلب افراد به طرق مختلف و به میزان وسیعی در معرض آن هستند. هدف: هدف از این تحقیق تعیین اثرات منفی فنل بر باروری موش سوری نر از طریق بررسی پارامترهای تحرک، قدرت زنده ماندن و تولید روزانه اسپرم میباشد. روش کار: 106 موش سوری نژاد NIH به سه گروه تیمار و یک گروه کنترل 13 تایی تقسیم شدند. گروه‌‌های تیمار 35روز روزانه با دوزهای mg/kg‌30 ،75و 100 گاواژ شدند و گروه کنترل نرمال سالین دریافت کرد. در روز 36، 6 موش نر از هر گروه آسان کشی شده و ایندکس گونادوسوماتیک، قدرت زنده ماندن، تحرک، و تولید روزانه اسپرم به دقت تعیین گردید. 7 موش باقیمانده از هر گروه، هر کدام با دو موش ماده جفت شدند. در روز دهم آبستی،موشها بیهوش و میزان باروری تعیین شد. نتایج: تولید روزانه اسپرم در گروههای تیمار نسبت به گروه کنترل کاهش معنیداری داشت (0.05>p). وزن بدن، تحرک، قدرت زنده ماندن و درصد باروری در بین گروهای 75، 100 با گروه کنترل دارای کاهش معنیداری بود (0.05>p). در حالیکه تغییرات وزن بیضه در هیچ یک از گروهها معنیدار نبود. ایندکس گونادوسوماتیک در گروههای تیمار نسبت به گروه کنترل افزایش معنیداری داشت (0.05>p). نتیجهگیرینهایی: فنل خالص با کاهش تحرک و تولید روزانه و قدرت زنده ماندن اسپرم میتواند میزان باروری را کاهش دهد.

Keywords [Persian]

  • باروری
  • فنل
  • موش سوری
  • اسپرم
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