Investigation on mycoflora of poultry breeding houses’ air and studying the efficacy of spraying and fumigation on inactivating the airspora

Document Type : Infectious agents- Diseases

Author

Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

Abstract

Background: A common concern of the poultry industry is the presence of fungal pathogens in the birds’ environment, which may constitute a considerable health hazard to the birds, farmers, and those living in proximity of the farm. OBJECTIVES: The aims of this study were to assess the mycoflora in the indoor and outdoor environments of poultry breeding houses and studying the efficacy of disinfection methods, including spraying and fumigation, on reducing airspora concentration. METHODS: Indoor air of 12 poultry houses were sampled by exposing Petri dishes containing Sabouraud’s glucose agar after removal of old litter, spraying with disinfectant solutions, and fumigation with formalin plus permanganate. The plates were incubated at 30 °C for seven days and fungi were counted and identified microscopically and macroscopically according to standard mycological methods. RESULTS: A total of 182 and 181 fungal colonies were recovered from indoor and outdoor air of poultry houses, respectively. Candida (30.2%) and Aspergillus (26.9%) species were the most common yeast and mold in the indoor, respectively, whereas Alternaria (37.6%) and Candida (19.3%) species were the most predominant fungi in the outdoor air of poultry houses. Disinfection of the poultry houses using spraying and fumigation methods led to a 38.1% and 75% reduction in airspora concentration (p<0.05), respectively. CONCLUSIONS: Based on the findings of the present study, Candida spp and Alternaria spp had the highest indoor and outdoor concentrations in poultry breeding houses’ air, respectively, and fumigation was the most efficient method in reducing airspora.

Keywords

Article Title [Persian]

بررسی فلور قارچی هوای سالن های پرورش طیور و مطالعه تأثیر اسپری کردن و دوددهی بر غیرفعال سازی اسپورهای هوا

Author [Persian]

  • حجت اله شکری
گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه تخصصی فناوریهای نوین آمل، آمل، ایران
Abstract [Persian]

زمینه مطالعه: یک نگرانی شایع در صنعت مرغداری حضور عوامل بیماریزای قارچی در محیط پرندگان است که خطر قابل ملاحظه‌ای برای پرندگان، کارگران مرغداری و تمام افرادی است که در نزدیکی مرغداری زندگی می‌کنند. هدف: اهداف این مطالعه تشخیص فلور قارچی در محیط‌های داخل و خارج سالن‌های پرورش طیور و مطالعه تأثیر روش‌های ضدعفونی کردن اسپری و دوددهی روی کاهش غلظت اسپورهای هوا بودند. مواد و روش کار: هوای داخلی 12 سالن مرغداری با روش مواجهه پتری دیش‌های حاوی سابوروگلوکز آگار  بعد از برداشت پوشال قدیمی، اسپری کردن با محلول‌های ضدعفونی کننده و دوددهی با فرمالین بعلاوه پرمنگنات نمونه‌برداری شدند. پلیت‌ها در حرارت C°30 بمدت 7 روز گرمخانه‌گذاری شدند و قارچ‌ها به‌صورت میکروسکوپی و ماکروسکوپی مطابق روش‌های استاندارد قارچ‌شناسی شمارش و شناسایی شدند. نتایج: در مجموع 182 و 181 کولونی قارچی بترتیب از هوای داخل و خارج سالن‌های مرغداری بدست آمدند. گونه‌های کاندیدا (2/30%) و آسپرژیلوس (9/26%) به‌ترتیب شایع‌ترین قارچ‌های مخمری و رشته‌ای در هوای داخل سالن بودند، در حالیکه گونه‌های آلترناریا (6/37%) و کاندیدا  (3/19%) بعنوان برجسته‌ترین قارچ‌ها در هوای خارج سالن‌های مرغداری شناسایی شدند. ضدعفونی سالن‌های مرغداری با استفاده از روش‌های اسپری و دوددهی به‌ترتیب موجب کاهش 1/38% و 75% در غلظت اسپورهای هوا شدند (0.05>p). نتیجه‌گیری‌نهایی: در مجموع، گونه‌های کاندیدا و آلترناریا به‌ترتیب بیشترین غلظت را در هوای داخل و خارج سالن‌های مرغداری داشتند و بیشترین تأثیر روی کاهش اسپورهای هوا مربوط به روش دوددهی بود. 

Keywords [Persian]

  • اسپورهای هوا
  • کاندیدا
  • دوددهی
  • فلور قارچی
  • سالن مرغداری

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