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Iranian Journal of Veterinary Medicine
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Afshari, A., Rad, M., Seifi, H., Ghazvini, K. (2016). Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR. Iranian Journal of Veterinary Medicine, 10(1), 33-40. doi: 10.22059/ijvm.2016.57048
Asma Afshari; Mehrnaz Rad; Hesamedin Seifi; Kiarash Ghazvini. "Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR". Iranian Journal of Veterinary Medicine, 10, 1, 2016, 33-40. doi: 10.22059/ijvm.2016.57048
Afshari, A., Rad, M., Seifi, H., Ghazvini, K. (2016). 'Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR', Iranian Journal of Veterinary Medicine, 10(1), pp. 33-40. doi: 10.22059/ijvm.2016.57048
Afshari, A., Rad, M., Seifi, H., Ghazvini, K. Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR. Iranian Journal of Veterinary Medicine, 2016; 10(1): 33-40. doi: 10.22059/ijvm.2016.57048

Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR

Article 5, Volume 10, Issue 1, Winter 2016, Page 33-40  XML PDF (1.44 MB)
Document Type: Infectious agents- Diseases- Surgery
DOI: 10.22059/ijvm.2016.57048
Authors
Asma Afshari1; Mehrnaz Rad email 1; Hesamedin Seifi2; Kiarash Ghazvini3
1Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2Center of Excellence in Ruminant Abortion and Neonatal Mortality and Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi Unversity of Mashhad, Mashhad, Iran
3Antimicrobial Resistance Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Background: Various strains of Escherichia coli (E. coli) are known as major causes of intestinal and extraintestinal infections in humans and various animal species. Molecular methods are important for the identification of bacterial isolates and nucleotide sequence variations, as well as information on tracking bacterial agents related to the outbreaks, the frequency of the bacterial genetic structure, and the evolution of microbial populations. Objectives: The purpose of the present study was to evaluate the efficiency of the RAPD method to differentiate E. coli strains. Methods: In this study, 110 isolates of E. coli were analyzed by the RAPD PCR method using two 10bp oligonucleotides. These strains were isolated from humans with urinary tract infections and neonatal calves affected by diarrhea or septicemia. Results: Data analysis showed that 87.5% of human E. coli isolates were correctly classified in the human host group, while 94.3% of calf E. coli isolates were correctly placed in calf groups. It also demonstrated that 100% and 93.3% of isolates were accurately assigned to diarrheic and septicemic calf groups, respectively. ConclusionS: Genetic variation analysis indicated that the percentage of polymorphism among E. coli isolates from humans with urinary tract infections, diarrheic calves, and septicemic neonatal calves were 54.71%, 61.22%, and 62.5%, respectively.
Keywords
E. coli; Genetic variation; RAPD-PCR
Article Title [Persian]
تنوع ژنتیکی جدای های اشریشیا کلی از انسان و گوساله ها به روش RAPD-PCR
Authors [Persian]
اسما افشاری1; مهرناز راد1; حسام الدین سیفی2; کیارش قزوینی3
1گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
2قطب علمی مطالعات سقط جنین و مرگ و میر نوزاد دامهای نشخوارکننده و گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
3مرکز تحقیقات مقاومت آنتی بیوتیکی، دانشکده پزشکی دانشگاه علوم پزشکی مشهد، مشهد، ایران
Abstract [Persian]
سویه‌های مختلف اشریشیا کلی به عنوان عوامل مهم عفونت‌های داخل و خارج روده‌ای در انسان و گونه‌های مختلف دامی شناخته می‌شوند. روش‌های مولکولی، برای شناسایی جدایه‌های باکتریایی، تنوع سکانس نوکلئوتیدی، اطلاعاتی در زمینه ردیابی عوامل باکتریایی دخیل در همه گیری‌ها، شناسایی تکراردر ساختار ژنتیکی و نیز شناسایی تکامل تدریجی باکتری‌ها، اهمیت دارند. هدف: هدف از این مطالعه ارزیابی میزان کارایی روش RAPD-PCR در تفکیک جدایه‌های اشریشیا کلی می‌باشد. روش‌کار: در این مطالعه 110 جدایه اشریشیا کلی از منابع مختلف به کمک دو الیگونوکلئوتید bp‌10 با سکانس‌های اختیاری به روش RAPD-PCR مورد بررسی قرار گرفتند. این جدایه‌ها از افراد مبتلا به عفونت ادراری، گوساله‌های نوزاد مبتلا به اسهال یا سپتی سمی، جدا شده بودند. نتایج: تجزیه و تحلیل اطلاعات نشان داد که 5/87٪ ازجدایه‌های اشریشیا کلی جدا شده از انسان به درستی در گروه میزبان انسان طبقه‌بندی شدند، درحالی که 3/94٪ از جدایه‌های اشریشیا کلی به درستی در گروه‌های مربوط به گوساله قرار گرفتند. همچنین نشان داده شد که 100٪ و 3/93٪ از جدایه‌ها به درستی به ترتیب درگروه‌های گوساله اسهالی وسپتی سمی قرار گرفتند. نتیجه‌گیری‌نهایی: آنالیز تنوع ژنتیکی نشان داد که درصد پلی مورفیسم در بین جدایه‌های اشریشیا کلی از افراد مبتلا به عفونت ادراری، گوساله‌های مبتلا به اسهال و گوساله‌های سپتی سمی به ترتیب 71/54٪، 22/61٪ و 5/62 ٪ بودند.
Keywords [Persian]
E. coli، تنوع ژنتیکی، RAPD-PCR
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