Detection of chlamydial infection in Iranian turkey flocks

Document Type : Infectious agents- Diseases


1 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Avian Diseases Faculty of Veterinary Medicine University of Tehran Tehran, Iran


Background: Avian chlamydiosis is a zoonotic disease of
birds caused by the intracellular bacterium Chlamydia psittaci. Avian
chlamydiosis leads to severe respiratory disease in young turkeys and egg
production losses in layers.
OBJECTIVES: Due to paucity of information about the
prevalence of chlamydial infection in the turkey population in Iran, this study
was conducted to detect chlamydial infection in some Iranian turkey flocks in
different provinces.
METHODS: A total of 177 samples were taken from turkeys
and first verified as Chlamydiaceae by Chlamydiaceae-specific
real-time polymerase chain reaction (real-time PCR) by detection of the 23S RNA
gene of Chlamydiaceae (Ct values ranging from 34 to 38) and then
positive samples were investigated for the presence of C. psittaci by a
nested PCR.
RESULTS: Seventeen of 177 samples (9.6%), corresponding
to 13 farms of 48 examined farms were positive for Chlamydiaceae by
real-time PCR. None of the positive samples were found to be C. psittaci
in the nested PCR.
CONCLUSIONS: This study showed no C. psittaci
infection in the turkey population in Iran. We recommend investigation on other
farm animals and wild populations for possible chlamydial infection and for
better understanding of the source and epidemiology of this agent. Due to the
challenges that exist for sampling and the relevant impact on reducing positive
samples, investigation by parallel and complementary techniques may be useful
in showing the true prevalence of infection in the target populations


Article Title [Persian]

جستجوی عفونت کلامیدیائی در گله های بوقلمون ایران

Authors [Persian]

  • زلیخا طاطاری 1
  • سید مصطفی پیغمبری 2
  • سید احمد مدنی 1
1 گروه بیماریهای طیور دانشکده دامپزشکی دانشگاه تهران تهران، ایران
2 گروه بیماریهای طیور دانشکده دامپزشکی دانشگاه تهران تهران، ایران
Abstract [Persian]

زمینه مطالعه:  کلامیدیوز پرندگان بیماری مشترک بین انسان و دام است که توسط باکتری داخل سلولی کلامیدیا پسیتاسی ایجاد می‌شود. کلامیدیوز پرندگان منجر به بیماریهای تنفسی شدید در بوقلمون‌های جوان و افت تولید تخم در گله‌های تخمگذار می‌گردد. هدف: با توجه به کمبود اطلاعات در مورد شیوع عفونت کلامیدیایی در جمعیت بوقلمون‌های ایران، این مطالعه با هدف تشخیص عفونت کلامیدیایی در برخی گله‌های بوقلمون ایران در استان های مختلف انجام گردید. روش کار: در مجموع 177 نمونه از بوقلمون‌ها گرفته شد و ابتدا بمنظور شناسایی خانواده کلامیدیاسه، تکنیک real- time PCR اختصاصی کلامیدیاسه که قطعه هدف آن ژن ریبوزومی 23S بود (Ct values بین 38-34) انجام گرفت و سپس نمونه‌های مثبت از نظر حضور کلامیدیا پسیتاسی با تکنیک nested PCR مورد بررسی قرار گرفتند. نتایج: هفده نمونه از 177 نمونه (%6/9) که متعلق به 13 فارم از 48 فارمی که مورد آزمایش قرار گرفته بودند در آزمون real-time PCR اختصاصی خانواده کلامیدیاسه مثبت بودند. هیچ یک از نمونه‌های مثبت، در آزمون nested PCR تشخیصی برای کلامیدیا پسیتاسی نتیجه مثبت را نشان ندادند. نتیجه گیری نهایی: در این مطالعه هیچگونه عفونت کلامیدیا پسیتاسی در جمعیت بوقلمون‌های ایران شناسایی نگردید. بررسی دیگر حیوانات اهلی و وحشی از نظر عفونت‌های محتمل کلامیدیایی، بمنظور درک بهتر منابع و اپیدمیولوژی این عامل توصیه می‌گردد. به علت چالش‌های موجود در نمونه‌گیری و اثرات آشکار آنها در کاهش نمونه‌های مثبت، استفاده از دیگر تکنیک‌های مکمل و همسو جهت نشان دادن شیوع دقیق آلودگی در جمعیت هدف، مناسب به نظر می‌رسد.

Keywords [Persian]

  • کلامیدیا پسیتاسی
  • real- time PCR
  • nested PCR
  • بوقلمون
Andersen, A.A., Vanrompay, D. (2000) Avian chlamydiosis. In: Diseases of Poultry. Saif, Y.M., Barnes, H.J., Fadly, A.M., Glisson, J.R., McDougald, L.R., Swayne, D.E., (eds.). (11th ed.) Iowa State University Press. Ames, Iowa, USA. p. 863-879.
Arraiz, N., Bermudez, V., Urdaneta, B., Mujica, E., Sanchez, M.P., Mejía, R., Prieto, C., Escalona, C., Mujica, A. (2012) Evidence of zoonotic Chlamydophila psittaci transmission in a population at risk in Zulia state, Venezuela, Rev Salud Pública. 14: 305-314.
Billington, S. (2005) Clinical and zoonotic aspects of psittacosis. In Pract. 27: 256-263.
Chahota, R., Ogawa, H., Mitsuhashi, Y., Ohya, K., Yamaguchi, T., Fukushi, H. (2006) Genetic diversity and epizootiology of Chlamydophila   prevalent among the captive and feral avian species based on VD2 region of ompA gene. Microbiol Immunol. 50: 663-678.
Christerson, L., Blomqvist, M., Grannas, K., Thollesson, M., Laroucau, K. Waldenstrom, J., Eliasson, I., Olsen, B., Herrmann, B. (2010) A novel Chlamydiaceae-like bacterium found in faecal specimens from sea birds from the Bering Sea. Environ Microbiol Rep. 2: 605-610.
DeGraves, F.J., Gao, D., Hehnen, H.R., Schlapp, T., Kaltenboeck, B. (2003) Quantitative detection of Chlamydia psittaci and C. pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle. J Clin Microbiol. 41: 1726-1729.
Doosti, A., Arshi, A. (2011) Determination of the Prevalence of Chlamydia psittaci by PCR in Iranian pigeons. Int J Biol. 3: 79-82.
Ehricht, R., Slickers, P., Goellner, S., Hotzel, H., Sachse, K. (2006) Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies. Mol Cell Prob. 20: 60-63.
Everett, K.D.E., Hornung, L.J., Andersen, A.A. (1999) Rapid detection of the Chlamydiaceae and other families in the order Chlamydiales: Three PCR tests. J Clin Microbiol. 37: 575-580.
Gaede, W., Reckling, K.F., Dresenkamp, B., Kenklies, S., Schubert, E., Noack, U., Irmscher, H.M., Ludwig, C., Hotzel, H., Sachse, K. (2008) Chlamydophila psittaci infections in humans during an outbreak of psittacosis from poultry in Germany. Zoonoses Pub Health. 55: 184-188.
Gasparini, J., Erin, N., Bertin, C., Jacquin, L., Vorimore, F., Frantz, A., Lenouvel, P., Laroucau, K. (2011) Impact of urban environment and host phenotype on the epidemiology of Chlamydiaceae in feral pigeons (Columbia livia). Environ Microbiol. 13: 3186-3193.
Geens, T., Desplanques, A., van Loock, M., Bönner, B.M., Kaleta, E.F. , Magnino, S., Andersen, A.A., Everett, K.D.E., Vanrompay, D. (2005a) Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method. J Clin Microbiol. 43: 2456-2461.
Geens, T., Dewitte, A., Boon, N., Vanrompay, D. (2005b) Development of a Chlamydophila psittaci species specific and genotype-specific real-time PCR. Vet Res. 36: 787-97.
Harkinezhad, T., Geens, T., Vanrompay, D. (2009) Chlamydophila psittaci infections in birds: a review with emphasis on zoonotic consequences. Vet Microbiol. 135: 68-77.
Herrmann, B., Rubaiyat, R., Bergström, S., Bonnedahl, J., Olsen, B. (2000) Chlamydophila abortus in a brown skua (Catharacta antarctica lonnbergi) from a subantarctic island. Appl Environ Microbiol. 66: 3654-3656.
Krieg, N.R., Parte, A., Ludwig, W., Whitman, W.B., Hedlund, B.P., Paster, B.J., Staley, J.T., Ward, N., Brown, D. (2010) Bergey’s Manual of Systematic Bacteriology. (2nd ed.) Vol. 4. Springer Verlag. New York, USA. p. 846.
Kuo, C., Stephens, R.S., Bavoil, P.M., Kaltenboeck, B. (2010) Chlamydia. In: Bergey’s Manual Systematic Bacteriology. (2nd ed.) Vol. 4. Springer Verlag. New York, USA. p. 846.
Laroucau, K., Vorimore, F., Aaziz, R., Berndt, A., Schubert, E., Sachse, K. (2009a) Isolation of a new chlamydial agent from infected domestic poultry coincided with cases of atypical pneumonia among slaughterhouse workers in France. Infect Gen Evol. 9: 1240-1247.
Laroucau, K., de Barbeyrac, B., Vorimore, F., Clerc, M., Bertin, C., Harkinezhad, T., Verminnen, K., Obeniche, F., Capek, I., Bébéar, C., Vanrompay, D., Garin-Bastuji, B., Sachse, K. (2009b) Chlamydial infections in duck farms associated with human cases of psittacosis in France. Vet. Microbiol. 135: 82-89.
Lemus, J.A., Fargallo, J.A., Vergara, P., Parejo, D., Banda, E. (2010) Natural cross chlamydial infection between livestock and free-living bird species. PLoS ONE. 5: e13512.
Madani, S.A., Peighambari, S.M., Barin, A. (2011) Isolation of Chlamydophila psittaci from pet birds in Iran. Int J Vet Res. 5: 95-98.
Madani, S.A., Peighambari, S.M. (2013) PCR-based diagnosis, molecular characterization and detection of atypical strains of avian Chlamydia psittaci in companion and wild birds, Avian Pathol. 42: 38-44.
Pantchev, A., Sting, R., Bauerfeind, R., Tyczka, J., Sachse, K. (2009) New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet J. 181: 145-150.
Sachse, K., Hotzel, H. (2003) Detection and differentiation of Chlamydiae by Nested-PCR. In: PCR Detection of Microbial Pathogens (Methods in Molecular Biology). Sachse, K., Frey, J. (eds.) Totowa: Humana Press. p. 123-136.
Sachse, K., Kuehlewind, S., Ruettger, A., Schubert, E., Rohde, G. (2012) More than classical Chlamydia psittaci in urban pigeons. Vet Microbiol. 157: 476-480.
Sachse, K., Laroucau, K., Hotzel, H., Schubert, E., Ehricht, R., Slickers, P. (2008) Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes. BMC Microbiol. 8: e63.
Vanrompay, D., Ducatelle, R., Haesebrouck, F. (1995a) Chlamydia psittaci infections: a review with emphasis on avian chlamydiosis. Vet Microbiol. 45: 93-119.
Vanrompay, D., Harkinezhad, T., Van De Walle, M., Beeckman, D., van Droogenbroeck, C., Verminnen, K., Leten, R., Martel, A., Cauwerts, K. (2007) Chlamydophila psittaci transmission from pet birds to humans. Emerg Infect Dis. 13: 1108-1110.
Vanrompay, D., Mast, J., Ducatelle, R., Haesebrouck, F., Goddeeris, B. M. (1995b) Chlamydia psittaci infections in turkeys: pathogenesis of infections in avian serovar A, B and D. Vet Microbiol. 47: 245-256.
van Loock, M., Geens, T., De Smit, L., Nauwynck, H., van Empe, P., Naylor, C., Hafez, H.M., Goddeeris, B.M., Vanrompay, D. (2005) Key role of Chlamydophila psittaci on Belgian turkey farms in association with other respiratory pathogens. Vet Microbiol. 107: 91-101.
Zocevic, A., Vorimore, F., Marhold, C., Horvatek, D., Wang, D., Slavec, B., Prentza, Z., Stavianis, G., Prukner-Radovcic, E., Dovc, A., Siarkou, V.I., Laroucau, K. (2012) Molecular characterization of atypical Chlamydia and evidence of their dissemination in different European and Asian chicken flocks by specific real-time PCR. Environ Microbiol. 8: 2212-2222.
Zocevic, A., Vorimore, F., Vicari, N., Gasparini, J., Jacquin, L., Sachse, K., Magnino, S., Laroucau, K. (2013) A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons. PLoS ONE. 8: e58741.