Detection of chlamydial infection in Iranian turkey flocks

Document Type: Infectious agents- Diseases- Surgery

Authors

1 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Avian Diseases Faculty of Veterinary Medicine University of Tehran Tehran, Iran

Abstract

Background: Avian chlamydiosis is a zoonotic disease of
birds caused by the intracellular bacterium Chlamydia psittaci. Avian
chlamydiosis leads to severe respiratory disease in young turkeys and egg
production losses in layers.
OBJECTIVES: Due to paucity of information about the
prevalence of chlamydial infection in the turkey population in Iran, this study
was conducted to detect chlamydial infection in some Iranian turkey flocks in
different provinces.
METHODS: A total of 177 samples were taken from turkeys
and first verified as Chlamydiaceae by Chlamydiaceae-specific
real-time polymerase chain reaction (real-time PCR) by detection of the 23S RNA
gene of Chlamydiaceae (Ct values ranging from 34 to 38) and then
positive samples were investigated for the presence of C. psittaci by a
nested PCR.
RESULTS: Seventeen of 177 samples (9.6%), corresponding
to 13 farms of 48 examined farms were positive for Chlamydiaceae by
real-time PCR. None of the positive samples were found to be C. psittaci
in the nested PCR.
CONCLUSIONS: This study showed no C. psittaci
infection in the turkey population in Iran. We recommend investigation on other
farm animals and wild populations for possible chlamydial infection and for
better understanding of the source and epidemiology of this agent. Due to the
challenges that exist for sampling and the relevant impact on reducing positive
samples, investigation by parallel and complementary techniques may be useful
in showing the true prevalence of infection in the target populations

Keywords


Article Title [Persian]

جستجوی عفونت کلامیدیائی در گله های بوقلمون ایران

Authors [Persian]

  • زلیخا طاطاری 1
  • سید مصطفی پیغمبری 2
  • سید احمد مدنی 1
1 گروه بیماریهای طیور دانشکده دامپزشکی دانشگاه تهران تهران، ایران
2 گروه بیماریهای طیور دانشکده دامپزشکی دانشگاه تهران تهران، ایران
Abstract [Persian]

زمینه مطالعه:  کلامیدیوز پرندگان بیماری مشترک بین انسان و دام است که توسط باکتری داخل سلولی کلامیدیا پسیتاسی ایجاد می‌شود. کلامیدیوز پرندگان منجر به بیماریهای تنفسی شدید در بوقلمون‌های جوان و افت تولید تخم در گله‌های تخمگذار می‌گردد. هدف: با توجه به کمبود اطلاعات در مورد شیوع عفونت کلامیدیایی در جمعیت بوقلمون‌های ایران، این مطالعه با هدف تشخیص عفونت کلامیدیایی در برخی گله‌های بوقلمون ایران در استان های مختلف انجام گردید. روش کار: در مجموع 177 نمونه از بوقلمون‌ها گرفته شد و ابتدا بمنظور شناسایی خانواده کلامیدیاسه، تکنیک real- time PCR اختصاصی کلامیدیاسه که قطعه هدف آن ژن ریبوزومی 23S بود (Ct values بین 38-34) انجام گرفت و سپس نمونه‌های مثبت از نظر حضور کلامیدیا پسیتاسی با تکنیک nested PCR مورد بررسی قرار گرفتند. نتایج: هفده نمونه از 177 نمونه (%6/9) که متعلق به 13 فارم از 48 فارمی که مورد آزمایش قرار گرفته بودند در آزمون real-time PCR اختصاصی خانواده کلامیدیاسه مثبت بودند. هیچ یک از نمونه‌های مثبت، در آزمون nested PCR تشخیصی برای کلامیدیا پسیتاسی نتیجه مثبت را نشان ندادند. نتیجه گیری نهایی: در این مطالعه هیچگونه عفونت کلامیدیا پسیتاسی در جمعیت بوقلمون‌های ایران شناسایی نگردید. بررسی دیگر حیوانات اهلی و وحشی از نظر عفونت‌های محتمل کلامیدیایی، بمنظور درک بهتر منابع و اپیدمیولوژی این عامل توصیه می‌گردد. به علت چالش‌های موجود در نمونه‌گیری و اثرات آشکار آنها در کاهش نمونه‌های مثبت، استفاده از دیگر تکنیک‌های مکمل و همسو جهت نشان دادن شیوع دقیق آلودگی در جمعیت هدف، مناسب به نظر می‌رسد.

Keywords [Persian]

  • کلامیدیا پسیتاسی
  • real- time PCR
  • nested PCR
  • بوقلمون

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