Detection of eaeA, hlyA, stx1 and stx2 genes in pathogenic Escherichia coli isolated from broilers affected with colibacillosis

Document Type : Infectious agents- Diseases

Authors

1 دانشگاه فردوسی مشهد

2 Faculty of veterinary medicine, ferdowsi University of Mashhad, Mashhad, Iran

3 Ferdowsi University of Mashhad

Abstract

Background: Foodborne outbreaks associated with shiga toxin-producing Escherichia coli (STEC) have been well documented worldwide. STECs are major causative agents of gastroenteritis in humans that may be complicated by hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), and thrombotic thrombocytopenia purpura (TTP). OBJECTIVES: The aim of this study was to investigate the presence of virulence-associated genes including eaeA, hlyA, stx1 and stx2 in Escherichia coli strains isolated from broiler’s Colibacillosis in the northeast of Iran. METHODS: From a total of 78 E.coli strains isolated from yolk sac infection, hepatitis and pericarditis, that were referred to educational veterinary clinic during 2011-2014,  subculturing of the isolates was performed using selective media and a typical colony from each sample was subjected to multiplex PCR assay for identification of the presence of STEC important virulence-associated genes (eaeA, hlyA,stx1 and stx2) causing shiga toxin-mediated diseases.                                                                                                                                       RESULTS: Of 78 E.coli isolates, one isolate was positive for both eaeA and hlyA genes while negative for stx1 and stx2 genes. CONCLUSIONS: The results showed low prevalence of STEC virulence genes associated with human infections in avian pathogenic E.coli (APEC) strains isolated from different flocks of broilers affected with colibacillosis.
 

Keywords

Article Title [Persian]

جستجوی فاکتور های حدت سویه های وروتوکسیژنیک اشریشیا کولای جدا شده از نیمچه های گوشتی شمال شرق ایران

Authors [Persian]

  • عبداله جمشیدی 1
  • جمشید رزمیار 2
  • ندا فلاح 3
1 گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
2 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
3 گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی دانشگاه فردوسی مشهد، مشهد، ایران
Abstract [Persian]

زمینه مطالعه:  شیوع بیماریهای غذا زاد ناشی از اشریشیا کولای تولید کننده شیگا توکسین در سراسر جهان کاملاً شناخته شده است. این توکسینها عوامل عمده گاسترو انتریتهای جوامع انسانی می‌باشند که گاها با کولیت خونریزی دهنده، سندرم اورمیک-همولیتیک و پورپورا ترمبو سایتو پنی پیچیده تر نیز می‌گردد. هدف: هدف این مطالعه بررسی حضورفاکتور‌های حدت شامل eaeA، hlyA، stx1 و stx2در سویه‌های اشریشیا کولای جداشده از نیمچه‌های گوشتی مبتلا به کلی باسیلوزدر شمال شرق ایران بود. روش‌کار: تعداد 78 سویه اشریشیا کولای از موارد هپاتیت و پریکاردیت نیمچه‌های گوشتی ارجائی به کلینیک آموزش دانشکده در طی سال‌های 1390-1393 جداسازی گردید. این سویه‌ها با استفاده از محیط‌های انتخابی کشت مجدد گردیده و یک کلنی تیپیک از هر نمونه به روش PCR چند گانه از نظر حضور ژن‌های حدت STECر(eaeA، hlyA، stx1 و stx2) مؤثر در بیماریهای ناشی از شیگا توکسین مورد بررسی قرار گرفت. نتایج: از تعداد 78 سویه جداشده اشریشیا کولای، تنها یک ایزوله واجد ژن‌های eaeA و hlyA بود که فاقد ژن‌های stx1 و stx2  بود. نتیجه گیری نهایی: نتایج این بررسی نشان دهنده شیوع پایین ژن‌های حدت سویه‌های ورو توکسیژنیک در اشریشیا کولای جدا شده از نیمچه‌های گوشتی مبتلا به کلی باسیلوزیس می‌باشد.

Keywords [Persian]

  • کلید واژگان: اشریشیا کولای
  • شیگا توکسین
  • کلی باسیلوز

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Iranian Journal of Veterinary Medicine
Volume 10, Issue 2
April 2016
Pages 97-103

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