EPSA1 and VPF genes expression during embryonic and larval development period of Beluga, Huso huso

Document Type : Genetics


Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


Background: The Endothelial PAS domain-containing protein 1 (EPSA1) is the key transcriptional regulator of hypoxic response and Vascular Permeability Factor (VPF) is an important growth factor for vascular development and angiogenesis. OBJECTIVES: In the present study, the levels of the EPSA1 coding gene and VPF transcripts were evaluated during Larval development of Beluga, Huso huso. METHODS: Samples at 12 developmental time-points including 1, 2, 4 days before hatch (eyed eggs), fresh hatched larvae (0), and larvae 1, 3, 6, 10, 15, 20, 25 and 50 days post-hatching were collected and  stored in a −80 °C freezer until RNA extraction. Changes in EPSA1 and VPF mRNA expression were studied and differences in normalized mRNA expression levels among the different developmental stages of H. huso were analyzed by one-way analysis of variance (ANOVA). RESULTS: The transcripts of EPSA1 and VPF were detected in all developmental time-points of H. huso from embryos to fingerling fish. Our results revealed that the mRNA expression of EPSA1 and VPF was low during embryonic development and then upregulated significantly at the time of hatch and early larval time-points, whereas in the late larval development stages they started to decline. CONCLUSIONS: This study showed that there is an association between the EPSA1 and VPF mRNA expression during larval development of H. huso. The up regulation of EPSA1 and VPF transcripts at the time of hatch and during yolk sac fry development of H. huso is likely tied to the role of them in vasculogenesis and angiogenesis.


Article Title [فارسی]

بیان ژنهای EPSA1 و VPF در طی دوره تکامل جنینی و لاروی فیل ماهی (Huso huso)

Author [فارسی]

  • علی طاهری میرقائد
گروه بهداشت و بیماریهای آبزیان، دانشکده دامپرشکی دانشگاه تهران، تهران، ایران
Abstract [فارسی]

زمینه مطالعه: ژن EPSA1ر(1 Endothelial PAS domain-containing protein)  یک تنظیم کننده رونویسی تحت تأثیر شرایط
هایپوکسی بوده و ژن VPFر(Vascular Permeability Factor)
بعنوان یک فاکتور مهم برای رشد و تکامل عروق خونی می‌باشد. هدف: در
این مطالعه سطوح بیان ژن‌های EPSA1 و VPF در
دوره تکامل لاروی فیل ماهی مورد بررسی قرار گرفت. روش کار:  نمونه برداری از 12 مرحله تکاملی شامل 1، 2 و 4
روز قبل از تفریخ تخم، لارو های تازه تفریخ شده و لاروهای 1، 3، 6، 10، 15، 20، 25
و 50 روز پس از تفریخ  صورت پذیرفت. نمونه‌ها تا زمان انجام
مراحل کار و استخراج RNA در
فریزرoC‌80-  نگهداری شدند. سپس
تغییرات میزان بیان ژن‌های EPSA و VPF  مورد مطالعه قرار گرفت و تفاوت بین بیان ژن‌های مذکور در مراحل
مختلف تکاملی توسط آنالیز واریانس یک طرفه (one-way
ANOVA) مورد بررسی قرار گرفت. نتایج: بیان ژن‌های ژن‌های EPSA و VPF در
تمامی مراحل تکاملی جنینی و مراحل ابتدایی لاروی صورت پذیرفت. نتایج نشان داد که
بیان ژن‌های ژن‌های EPSA و VPF  در دوره جنینی پایین بوده  و سپس در زمان تفریخ و مراحل ابتدایی تکامل
لاروی افزایش در بیان مشاهده شد  درحالیکه
با افزایش سن لارو بیان ژن‌های مذکور روند
رو  به کاهش داشت. نتیجه گیری نهایی:
این مطالعه نشان می‌دهد که ارتباط
تنگاتنگی بین ژن‌های ژن‌های EPSA و VPF در
مراحل تکامل لاروی فیل ماهی وجود دارد و افزایش بیان ژن‌ها مذکور در مراحل
ابتدای تفریخ و مراحل تکاملی لاروی جذب زرده فیل ماهی،  نشان دهنده اهمیت آن در تکامل رگزایی می‌باشد

Keywords [فارسی]

  • بیان ژن
  • تکامل لاروی
  • ماهی خاویاری
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