Identification of enterototxin harboring gene among Clostridium perfringens isolates with different toxin types in Iran

Document Type : Infectious agents- Diseases

Authors

1 Razi vaccine and Serum Research Institute

2 Razi vaccine and Serum Research Inst

3 Payame Noor University, Tehran Branch,

Abstract

Background: Clostridium perfringens is known as the most widely distributed pathogenic microorganism in nature. It is an extremely important pathogen of human and domestic animals. In a commonly used classification scheme, C. perfringens is divided into five toxinotypes (A to E) based on the production of four major toxins (alpha, beta, epsilon, and iota). Enterotoxin is not usually used for C. perfringens typing but it is a fatal toxin with necrotic activity. Based on our knowledge there is no published scientific report regarding identification of enterotoxin positive C. perfringens isolates from animals in Iran. OBJECTIVES: To study the presence and frequency of enterotoxin gene among C. perfringence isolates with different types. METHODS: A specific single PCR assay was developed and used for detection of cpe gene to identify the entrerotoxin  harboring isolates among different types of C. perfringens isolated from animal enteric diseases in Iran. RESULTS: It was found that cpe gene presents among C.perfringens isolates types A, B, C  and D with  63.6% (7/11), 25%  ( 5/20),  21.4% (3/14), 53.3% (8/15),  respectively. Totally 23 of 60 ( 38.3%) isolates screened by PCR were cpe-positive. CONCLUSIONS: This is the first report of cpe- positive isolates of C.perfringens causing enterototoxemia  in animals in Iran. Further studies to investigate the synergistic effect of CPE toxin in pathogenesis of enteric diseases in animals is suggested.
 

Keywords

Article Title [Persian]

شناسایی جدایه های کلستریدیوم پرفرنجنس واجد ژن آنتروتوکسین در بین تیپ های توکسینی مختلف از ایران

Authors [Persian]

  • احمدرضا جباری 1
  • مجید اسماعیل زاد 2
  • فریبا صمیمی 3
1 موسسه تحقیقات واکسن و سرم سازی رازی
2 آزمایشگاه مرکزی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران
3 دانشگاه پیام نور واحد تهران، تهران، ایران
Abstract [Persian]

 
زمینه مطالعه: کلستریدیوم پرفرنجنس به عنوان میکروارگانیسم بیماریزا با انتشار وسیع شناخته شده است. این باکتری در انسان و حیوانات بیماریزایی بسیار مهمی دارد. در یک طبقه بندی کلاسیک کلستریدیوم پرفرنجنس در پنج تیپ توکسینی (از A تا E) بر مبنای ترشح چهار توکسین اصلی (آلفا، بتا، اپسیلون و یوتا) قرار می‌گیرد. آنتروتوکسین به طور معمول در تایپینگ کلستریدیوم پرفرنجنس استفاده نمی‌شود اما یک توکسین کشنده توام با فعالیت نکروتیک محسوب می‌گردد. تاکنون گزارش علمی منتشر شده‌ای در خصوص شناسایی جدایه‌های کلستریدیوم پرفرنجنس از حیوانات در ایران وجود ندارد. هدف: در این تحقیق حضور ‍و فراوانی ژن آنتروتوکسین در بین جدایه‌های کلستریدیوم پرفرنجنس تیپ‌های مختلف مورد بررسی قرار گرفت. روش کار: آزمایش PCR اختصاصی به منظور شناسایی ژن آنتروتوکسین cpe در بین جدایه‌های کلستریدیوم پرفرنجنس جدا شده از مبتلایان به بیماریهای روده‌ای دام‌‌‌ها در ایران استفاده گردید. نتایج: نتایج نشان داد که ژن cpe در جدایه‌های کلستریدیوم پرفرنجنس تیپ‌های A، B، C، D به ترتیب با فراوانی 63% (11/7)، 25% (20/5)، 4/21% (14/3) و 3/53 (15/8) حضور دارد. در مجموع 23 جدایه از 60 جدایه مطالعه شده (3/31%) با PCR از نظر حضور ژن cpe مثبت بودند. نتیجه گیری نهایی:  این اولین گزارش از شناسایی موارد cpe مثبت در بین جدایه‌ای کلستریدیوم پرفرنجنس عامل آنتروتوکسیمی در دام‌ها در ایران می باشد. مطالعات تکمیلی به منظور بررسی میزان تأثیر افزایشی توکسین cpe در پاتوژنز بیماریهای روده‌ای حیوانات پیشنهاد می شود.

Keywords [Persian]

  • کلستریدیوم پرفرنجنس
  • ژن cpe
  • آنتروتوکسین

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Iranian Journal of Veterinary Medicine
Volume 10, Issue 3
September 2016
Pages 165-172

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