Antifungal activity of the Trachyspermum ammi essential oil on some of the most common fungal pathogens in animals

Document Type : Infectious agents- Diseases


1 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.


Background: The increasing resistance to antifungal drugs and the reduced number of available drugs led to the search for therapeutic alternatives among aromatic plants and their essential oils, empirically used by antifungal effects. OBJECTIVES: The purpose of the current study was to evaluate the antifungal activity of Trachyspermum ammi essential oil (EO) against the most frequent pathogenic fungi including Candida, Aspergillus, Chrysosporium and Trichophyton species. METHODS: EO from the seeds of the plant was obtained by hydrodistillation. Susceptibility tests were expressed as growth inhibition zone (diameter) using disk diffusion method and minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) using broth microdilution method. RESULTS: Results of susceptibility tests showed that T. ammi EO was effective against all the tested strains. The diameters of growth inhibition zone of the EO were between 11 mm and 60 mm. The EO was also the most active, with MIC and MFC values ranging from 0.3 to 2.5 mg/ml and 0.6 to 5 mg/ml, respectively. The EO of T. ammi showed a significant degree of antifungal activity against different Candida species in comparison with other fungi (p<0.05). CONCLUSIONS: The present study indicated that T. ammi EO has considerable antifungal activity, deserving further investigations for its clinical application for treatment of fungal infections.


Article Title [Persian]

فعالیت ضدقارچی اسانس روغنی زنیان بر روی تعدادی از شایع‌ترین عوامل بیماری‌زای قارچی در حیوانات

Authors [Persian]

  • حجت اله شکری 1
  • عقیل شریف زاده 2
  • علیرضا خسروی 2
1 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه تخصصی فناور یهای نوین آمل، آمل، ایران
2 مرکز تحقیقات قارچ شناسی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
Abstract [Persian]

زمینه مطالعه: مقاومت رو به رشد نسبت به داروهای ضدقارچی و کاهش تعداد داروهای موجود سبب شد تا بررسی در زمینه داروهای جایگزین درمانی میان گیاهان آروماتیک و اسانسهای روغنیشان بهویژه با اثرات ضدقارچی انجام شود. هدف: هدف مطالعه حاضر، ارزیابی فعالیت ضدقارچی اسانس روغنی زنیان (Trachyspermum ammi) در برابر شایعترین قارچهای بیماریزا نظیر گونههای کاندیدا، آسپرژیلوس، کرایزوسپوریوم و ترایکوفایتون بود. روش کار: اسانس روغنی از دانههای گیاه با روش تقطیر آبی به دست آمد. آزمایشهای حساسیت به صورت هالة مهار رشد (قطر) با روش دیسک دیفیوژن و حداقل غلظت مهاری (MIC) و حداقل غلظت قارچکشی (MFC) با روش میکرودایلوشن براث بیان شدند. نتایج: نتایج آزمایشهای حساسیت نشان دادند که روغن زنیان در مقابل تمام سویههای آزمایش شده فعال بود. قطرهای هالة مهار رشد روغن بین mm‌11 و 60 بودند. همچنین روغن به ترتیب با مقادیر MIC و MFC بین mg/ml 3/0 تا 5/2 و mg/ml 6/0 تا 5 بسیار مؤثر بود. اسانس روغنی زنیان فعالیت معنیداری را در مقابل گونههای مختلف کاندیدا در مقایسه با سایر قارچها نشان داد (0.05>p). نتیجهگیری نهایی: مطالعه حاضر نشان داد که اسانس روغنی زنیان فعالیت قابل ملاحظه ضدقارچی دارد و شایسته است تحقیقات گستردهتری برای کاربرد بالینی آن جهت درمان عفونتهای قارچی انجام پذیرد.

Keywords [Persian]

  • فعالیت ضدقارچی
  • آسپرژیلوس
  • کاندیدا
  • زنیان
  • ترایکوفایتون
Alcazar-Fuoli, E. (2013) Ergosterol biosynthesis in Aspergillus fumigatus: its relevance as an antifungal target and role in antifungal drug resistance. Front Microbiol. 3: 1-6.
Aligiannis, N., Kalpoutzakis, E., Mitaku, S. (2001) Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem. 38: 4168-4170.
Cavaleiro, C., Pinto, E., Goncalves, M.J. (2006) Antifungal activity of Juniperus essential oils against dermatophyte, Aspergillus and Candida strains. J Appl Microbiol. 100: 1333-1338.
Clinical and Laboratory Standards Institute (CLSI) (2008) Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard. (3rd ed.) CLSI document M27-A3, Clinical and Laboratory Standards Institute, Wayne, PA, USA.
Clinical and Laboratory Standards Institute (CLSI) (2008) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; Approved standard. (2nd ed.)CLSI document M38-A2, Clinical and Laboratory Standards Institute, Wayne, PA, USA.
Clinical and Laboratory Standards Institute (CLSI) (2004) Method for antifungal disk diffusion susceptibility testing of yeasts; Approved guideline. CLSI document M44-A, Clinical and Laboratory Standards Institute, Wayne, PA, USA.
Clinical and Laboratory Standards Institute (CLSI) (2008) Method for antifungal disk diffusion susceptibility testing of filamentous fungi; Proposed guideline. CLSI document M51-P, Clinical and Laboratory Standards Institute, Wayne, P, USA.
Council of Europe (1997) Methods of Pharmacognosy. In: European Pharmacopoeia. (3rd ed.) European Department for the Quality of Medicines. Strasbourg, France. p. 121-122.
Ebrahimzadeh, H., Yamini, Y., Sefidkonc, F. (2003) Chemical composition of the essential oil and supercritical CO2 extracts of Zataria multiflora Boiss. Food Chem. 83: 357-361.
Gandomi, H., Abbaszadeh, S., Jebellijavan, A. (2014) Chemical constituents, antimicrobial and antioxidative effects of Trachyspermum ammi essential oil. J Food Process Pres. 38: 1690-1695.
Gauthier, G.M., Keller, N.P. (2013) Crossover fungal pathogens, the biology and pathogenesis of fungi capable of crossing kingdoms to infect plants and humans. Fungal Genet Biol. 8: 1-12.
Gersbach, P.V., Reddy, N. (2002) Non-invasive localization of thymol accumulation in Carum copticum (Apiaceae) fruits by chemical shift selective magnetic resonance imaging. Ann Bot. 90: 253-257.
Hammer, K., Carson, C., Riley, T. (2002) In-vitro activity of Melaleuca alternifolia (tea tree) oil against dermatophytes and other filamentous fungi. J Antimicrob Chemother. 50: 195-199.
Ishikawa, T., Sega, Y., Kitajima, J. (2001) Water-soluble constituents of Ajowan. Chem Pharmaceut Bull. 49: 840-844.
Khosravi, A.R., Shokri, H., Yahyaraeyat, R. (2006) Veterinary mycology. (1st ed.) Tehran University Press. Tehran, Iran.
Klich, M.A. (2002) Identification of common Aspergillus species. (1st ed.) Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands.
Larone, D.H. (2002) Medically Important Fungi. A guide to Identification. (4th ed.) ASM Press. Washington D.C., USA.
Murthy, P.S., Borse, B.B., Khanum, H. (2009) Inhibitory effects of Ajowan (Trachyspermum ammi) ethanolic extract on A. ochraceus growth and ochratoxin production. Turk J Biol. 33: 211-217.
Naeini, A., Khosravi, A.R., Chitsaz, M. (2009) Anti-Candida albicans activity of some Iranian plants used in traditional medicine. J Mycol Méd. 19: 168-172.
Naeini, A., Shokri, H. (2012) Chemical composition and in vitro antifungal activity of the essential oil from Cuminum cyminum against various Aspergillus strains. J Med Plants Res. 6: 1702-1706.
Nostro, A., Blanco, A.R., Cannatelli, M.A. (2004) Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol Lett. 230: 191-195.
Pina-Vaz, C., Rodrigues, A.G., Pinto, E. (2004) Antifungal activity of Thymus oils and their major compounds. J Eur Acad Dermatol. 18: 73-78.
Pinto, E., Palmeira, A., Salgueiro, L. (2003) Antifungal activity of oregano oils (Lippia graveolens and Origanum virens) on dermatophyte species. Clin Microbiol Infect. 9: 222-230.
Ranjan, B., Manmohan, S., Singh, S.R. (2012) Medicinal uses of Trachyspermum ammi: a review. Pharmacogn Rev. 6: 56-60.
Salgueiro, L.R., Pinto, E., Gonc alves, M.J. (2004) Chemical composition and antifungal activity of the essential oil of Thymbra capitata. Planta Med. 70: 572-575.
Shojaaddini, M., Moharramipour, S., Sahaf, B. (2008) Fumigant toxicity of essential oil from Carum copticum against Indian meal moth, Plodia interpunctella. J Plant Prot Res. 48: 411-419.
Shokri, H., Khosravi, A.R., Rad, M.A. (2010) Occurrence of Malassezia species in Persian and domestic short hair cats with and without otitis externa. J Vet Med Sci. 72: 293-296.
Shokri, H., Sharifzadeh, A., Ashrafi Tamai, I. (2012) Anti-Candida zeylanoides activity of some Iranian plants used in traditional medicine. J Mycol Méd. 22: 211-216.
Soković, M.D., Vukojević, J., Marin, P.D. (2009) Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities. Molecul. 14: 238-249.
Soni, S., Soni, U.N. (2014) In-vitro anti-bacterial and anti-fungal activity of select essential oils. Int J Pharm Pharm Sci. 6: 586-591.
Tiwari, T.N., Chansouria, J.P.N., Dubey, N.K. (2003) Antimycotic potency of some essential oils in the treatment of induced dermatomycosis of an experimental animal. Pharm Biol. 41: 351-356.
Vandeputte, P., Selene, F., Coste, A.T. (2012) Antifungal resistance and new strategies to control fungal infections. Int J Microbiol. 2012: 1-26.
World Health Organization (WHO). (2001) General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, WHO, Geneva, Switzerland.