Antifungal activity of the Trachyspermum ammi essential oil on some of the most common fungal pathogens in animals

Document Type: Infectious agents- Diseases- Surgery

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Abstract

Background: The increasing resistance to antifungal drugs and the reduced number of available drugs led to the search for therapeutic alternatives among aromatic plants and their essential oils, empirically used by antifungal effects. OBJECTIVES: The purpose of the current study was to evaluate the antifungal activity of Trachyspermum ammi essential oil (EO) against the most frequent pathogenic fungi including Candida, Aspergillus, Chrysosporium and Trichophyton species. METHODS: EO from the seeds of the plant was obtained by hydrodistillation. Susceptibility tests were expressed as growth inhibition zone (diameter) using disk diffusion method and minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) using broth microdilution method. RESULTS: Results of susceptibility tests showed that T. ammi EO was effective against all the tested strains. The diameters of growth inhibition zone of the EO were between 11 mm and 60 mm. The EO was also the most active, with MIC and MFC values ranging from 0.3 to 2.5 mg/ml and 0.6 to 5 mg/ml, respectively. The EO of T. ammi showed a significant degree of antifungal activity against different Candida species in comparison with other fungi (p<0.05). CONCLUSIONS: The present study indicated that T. ammi EO has considerable antifungal activity, deserving further investigations for its clinical application for treatment of fungal infections.
 

Keywords


Article Title [Persian]

فعالیت ضدقارچی اسانس روغنی زنیان بر روی تعدادی از شایع‌ترین عوامل بیماری‌زای قارچی در حیوانات

Authors [Persian]

  • حجت اله شکری 1
  • عقیل شریف زاده 2
  • علیرضا خسروی 2
1 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه تخصصی فناور یهای نوین آمل، آمل، ایران
2 مرکز تحقیقات قارچ شناسی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
Abstract [Persian]

زمینه مطالعه: مقاومت رو به رشد نسبت به داروهای ضدقارچی و کاهش تعداد داروهای موجود سبب شد تا بررسی در زمینه داروهای جایگزین درمانی میان گیاهان آروماتیک و اسانسهای روغنیشان بهویژه با اثرات ضدقارچی انجام شود. هدف: هدف مطالعه حاضر، ارزیابی فعالیت ضدقارچی اسانس روغنی زنیان (Trachyspermum ammi) در برابر شایعترین قارچهای بیماریزا نظیر گونههای کاندیدا، آسپرژیلوس، کرایزوسپوریوم و ترایکوفایتون بود. روش کار: اسانس روغنی از دانههای گیاه با روش تقطیر آبی به دست آمد. آزمایشهای حساسیت به صورت هالة مهار رشد (قطر) با روش دیسک دیفیوژن و حداقل غلظت مهاری (MIC) و حداقل غلظت قارچکشی (MFC) با روش میکرودایلوشن براث بیان شدند. نتایج: نتایج آزمایشهای حساسیت نشان دادند که روغن زنیان در مقابل تمام سویههای آزمایش شده فعال بود. قطرهای هالة مهار رشد روغن بین mm‌11 و 60 بودند. همچنین روغن به ترتیب با مقادیر MIC و MFC بین mg/ml 3/0 تا 5/2 و mg/ml 6/0 تا 5 بسیار مؤثر بود. اسانس روغنی زنیان فعالیت معنیداری را در مقابل گونههای مختلف کاندیدا در مقایسه با سایر قارچها نشان داد (0.05>p). نتیجهگیری نهایی: مطالعه حاضر نشان داد که اسانس روغنی زنیان فعالیت قابل ملاحظه ضدقارچی دارد و شایسته است تحقیقات گستردهتری برای کاربرد بالینی آن جهت درمان عفونتهای قارچی انجام پذیرد.

Keywords [Persian]

  • فعالیت ضدقارچی
  • آسپرژیلوس
  • کاندیدا
  • زنیان
  • ترایکوفایتون

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