Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran

Document Type : Aquatic health and Culture

Authors

1 1Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 PhD Candidate, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran

3 Full professor, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran

4 Assistant professor, Aquatic animal health expert, Directorate of health and control of aquatic animal diseases, Iranian veterinary organization

5 Department of microbiology, faculty of science, university of Tehran

Abstract

Background: Motile Aeromonas species cause heavy
mortalities in carp farms during spring and summer in Iran. Objectives: The aim of this study was to detect phenotypic
and genotypic characterization of motile Aeromonas species isolated from
diseased carps in some northern and southern provinces of Iran. Methods: A number of 40 samples from 22 fish farms were
collected. The identified motile Aeromonas species were sequenced and
phylogenetic tree was drawn by MEGA6 using UPGMA analysis. Results: A number of 19 bacterial isolates were
identified as motile Aeromonas sp. by biochemical tests, and the DNA
segments of 16S rRNA gene of all these strains gave 1200 bp after running on 1%
agarose electrophorus gel. Also, the sequencing results showed that the
bacterial samples were determined as A. hydrophila and A. veronii
biovar veronii. Conclusions: Phylogenetic analysis revealed that motile Aeromonas
strains in this study were separated in two clusters and four  genogroups with high similarities

Keywords

References

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Iranian Journal of Veterinary Medicine
Volume 10, Issue 3
September 2016
Pages 209-216

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