Molecular characterization of recent Iranian infectious bronchitis virus isolates based on S2 protein gene

Document Type : Infectious agents- Diseases

Authors

1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.

2 Department of Microbiology and immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran - IRAN

3 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, IRAN.

4 Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IRAN.

5 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Background: Avian infectious bronchitis (IB), with avian
infectious bronchitis virus (IBV) as the causing agent, is a ubiquitous endemic
disease of the chicken with devastating effects on its industry. A viral
membrane surface protein called S  not
only induces neutralizing antibodies but also plays an important role in virus
binding and entry to host cells. Technically, S1 protein gene sequencing also
helps greatly in IBV genotyping. OBJECTIVES: The aim of this study was
to characterize Iranian IBV based on S2 gene. METHODS: After RT-PCR
amplification, the S2 gene of nine Iranian IBV isolates were sequenced and then
compared with reference strains. RESULTS: The isolates were classified
into genotype I as Massachusetts like IB Vs, genotype VII which clustered into
two branches, VIIa (IS-1494 like IB viruses), and VIIb, and was related to QX-
like viruses and Genotype VIII as 793/B like IBVs. CONCLUSIONS: As far
as we know, this is the first S2-based classification study on Iranian IBV
isolates providing a firm experimental basis to correlate with genotypic
characterization

Keywords

References

Abro, S.H. (2012) Molecular characterization and detection of infectious bronchitis virus.Vet  Microbiol.  155: 237–246.##
Aghakhan, S., Abshar, N., Fereidouni, S.R.N., Marunesi, C., Khodashenas, M. (1994) Studies on avian viral infections in Iran. Arch Inst  Razi. 44: 1-10.##
Ammayappan, A., Vakharia, V.N. (2009) Complete nucleotide analysis of the structural genome of the infectious bronchitis virus strain md27 reveals its mosaic nature. Viruses. 1: 1166-1177.##
Bozorgmehri-Fard, M., Charkhkar, S., Hosseini, H. (2014) Detection of the chinese genotype of infectious bronchitis virus (QX-type) in Iran. Iranian J Virol. 7: 21-24.##
Callison, S.A., Jackwood, M.W., Hilt, D.A. (1999) Infectious bronchitis virus S2 gene sequence variability may affect S1 subunit specific antibody binding. Virus Genes. 19: 143-151.##
Callison, S.A., Jackwood, M.W., Hilt, D.A. (1999) Infectious bronchitis virus S2 gene sequence variability may affect S1 subunit specific antibody binding. Virus Genes. 19: 143-151.##
Hosseini, H., Bozorgmehri Fard, M.H., Charkhkar, S., Morshed, R. (2015) Epidemiology of avian infectious bronchitis virus genotypes in Iran (2010-2014). Avi Dis. 59: 431-5.##
Jackwood, M. W. (2012) Review of infectious bronchitis virus around the world. Avian Dis. 56: 634-641.##
Lee, C.-W., Hilt, D. A., Jackwood, M.W. (2003) Typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the S1 gene. J Vet  Diag  Invest. 15: 344-348.##
Mase, M., Inoue, T., Yamaguchi, S., Imada, T. (2009) Genetic diversity of avian infectious bronchitis viruses in Japan based on analysis of s2 glycoprotein gene. J Vet  Med Sci. 71: 287-291.##
Mo, M.-L., Hong, S.-M., Kwon, H.-J., Kim, I.-H., Song, C.-S., Kim, J.-H. (2013) Genetic diversity of spike, 3a, 3b and e genes of infectious bronchitis viruses and emergence of new recombinants in Korea. Viruses, 5, 550-567.##
Najafi, H., Langeroudi, A.G., Hashemzadeh, M., Karimi, V., Madadgar, O., Ghafouri, S.A., Maghsoudlo, H., Farahani, R.K. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol. 161: 1-10.##
Najafi, H., Madadgar, O., Jamshidi, S., Langeroudi, A.G., Lemraski, M.D. (2014) Molecular and clinical study on prevalence of feline herpesvirus type 1 and calicivirus in correlation with feline leukemia and immunodeficiency viruses. In: Vet Res Forum (Vol. 5, pp. 255): Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. ##
Seyfi Abad Shapouri, M., Mayahi, M., Assasi, K., Charkhkar, S. (2004) A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran. Acta Vet Hung. 52: 163-166.##
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 28: 2731-2739.##
Zulperi, Z.M., Omar, A.R., Arshad, S.S. (2009) Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia. Virus Genes. 38: 383-391.##
 
 
 
Iranian Journal of Veterinary Medicine
Volume 10, Issue 4
December 2016
Pages 245-252

Files

Share

How to cite

Statistics