Peppermint and Pennyroyal Essential Oil Effect on Performance, Rumen Microbial Population and Some Blood Parameters of Sheep

Document Type: Reproduction - Physiology


1 M.Sc. graduate of Animal physiology, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran

2 Department of Animal Science, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran


BACKGROUND: The use of antibiotics as feed additive in animal feeds due to the appearance of residues in milk and meat and their effects on human health has restricted. Two of essential oils with high potential for use in ruminant diet are Mentha piperita (peppermint) and Mentha pulegium (pennyroyal) essential oil. OBJECTIVE: This study was conducted to investigate the effects of essential oils of peppermint and pennyroyal on performance, ruminal microbial population and some blood parameters of sheep. METHODS: For this purpose, 9 Dallagh sheep were used in a change over design experiment at three 21-d periods (14 days as adaptation and 7 days for sample collection). Experimental treatments were 1) basal diet without additive (control), 2) basal diet + 110 mg/d Mentha piperita essential oil and 3) basal diet +110 mg/d Mentha pulegium essential oil. Sheep were kept in individual cages and had free access to food and water. Rumen fluid was collected before, 4 h and 8 h after morning feeding and a blood sample was obtained 3 h after morning feeding at last day of each period. RESULTS: Essential oils had no effect on performance, blood parameters, pH, ammonia, protozoa, and total viable bacterial count of rumen. Coliforms of rumen fluid significantly decreased at 4 h and increased 8 h after morning feeding following peppermint and pennyroyal supplementation, respectively (P<0.05). Acid lactic bacteria count was significantly higher at before and 4 h after morning feeding in pennyroyal treatment compared with other treatments. CONCLUSION: although essential oils of Mentha piperita and Mentha pulegium had some effects on rumen microbial population but had no significant effects on performance and blood metabolites of Dallagh sheep.


Article Title [Persian]

اثر روغن‌ اسانسی نعناع و پونه بر عملکرد، جمعیت میکروبی شکمبه و برخی فراسنجه‌های خونی گوسفند

Authors [Persian]

  • روناک محمدی 1
  • رضا راه چمنی 2
  • فرزاد قنبری 2
  • فریبا فریور 2
1 دانش آموخته فیزیولوژی دام، دانشکده کشاورزی و منابع طبیعی دانشگاه گنبدکاووس، گنبد کاووس، ایران
2 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران
Abstract [Persian]

زمینه مطالعه: استفاده از آنتی بیوتیک‌ها به‌عنوان افزودنی غذایی در دام‌ها به علت باقیماندن آنتی بیوتیک در شیر، گوشت و اثرات آن در سلامت انسان‌ها محدود شده است. روغن اسانسی نعناع و پونه پتانسیل بالایی برای استفاده در جیره نشخوارکنندگان دارند. هدف: این مطالعه به‌منظور بررسی اثرات روغن‌های اسانسی نعناع و پونه بر عملکرد، جمعیت میکروبی شکمبه و برخی فراسنجه‌های خونی گوسفند انجام شد. روش کار: بدین منظور، از 9 رأس گوسفند نر نژاد‌ دالاق در قالب طرح چرخشی در 3 دوره 21 روزه شامل 14 روز به‌‌‌‌عنوان دوره عادت‌پذیری و 7 روز نمونه‌برداری استفاده شد. تیمارهای آزمایشی شامل تیمار1 (شاهد)‌: جیره پایه (بدون اسانس نعناع و پونه)، تیمار2: جیره پایه + 110 میلی گرم در روز اسانس نعناع و تیمار3: جیره پایه + 110 میلی گرم در روز اسانس پونه بودند و گوسفندان در قفس‌‌های انفرادی بطور آزاد به آب و غذا دسترسی داشتند. به‌منظور تعیین جمعیت میکروبی، pH و ازت آمونیاکی، نمونه‌های مایع شکمبه قبل از خوراک‌دهی صبح، 4 و 8 ساعت بعد از خوراک‌دهی صبح جمع‌آوری شدند. خون‌گیری در پایان هر دوره از سیاهرگ گردنی صورت گرفت. نتایج: روغن‌های اسانسی نعناع و پونه تأثیر معنی‌داری بر عملکرد، متابولیت‌های خونی، pH، ازت آمونیاکی، تعداد پروتوزوآ و شمارش کلی باکتری‌های مایع شکمبه نداشتند. تعداد کلی‌فرم‌های شکمبه 4 ساعت بعد خوراک‌دهی صبح در تیمار نعناع کاهش معنی‌دار و 8 ساعت بعد خوراک‌دهی در تیمار پونه افزایش معنی‌داری نسبت به تیمار شاهد داشت(05/0>P). تعداد باکتری‌های تولید کننده اسیدلاکتیک در زمان قبل و 4 ساعت بعد از خوراک‌دهی صبح در تیمار پونه بیشتر از تیمارهای شاهد و نعناع بود. نتیجه گیری نهایی: در مجموع، روغن‌های اسانسی نعناع و پونه هر چند بر جمعیت میکروبی شکمبه موثر بودند ولی تاثیری بر عملکرد و فراسنجه‌های خونی گوسفندان دالاق نداشتند.

Keywords [Persian]

  • متابولیتهای خونی
  • روغن اسانسی نعناع
  • روغن اسانسی پونه
  • جمعیت میکروبی
  • گوسفند
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