Survey on Serum Amyloid A and Haptoglobin Concentrations in Normal or Sensitive to Rumen Acidosis Holstein Dairy Cows

Document Type : Immunology

Authors

1 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Animal Sciences, Isfahan University of Technology, Isfahan, Iran

3 Produce Manager at Sharif Abad Dairy Farm ,Qazvin,,Iran

Abstract

　
BACKGROUND: Diagnosis and prognosis of inflammatory conditions based on acute phase proteins (APPs) level in blood has been exploited for a long time in human medicine and their changes in SARA condition is considered in veterinary medicine.
OBJECTIVES:
To evaluate the variations of serum amyloid A (SAA) and haptoglobin (Hp) as main acute phase proteins in cow with normal pH or cows that experienced rumen pH ≤5.8 during first days after parturition.
METHODS:
A total of 106 multiparous Holstein dairy cows were randomly selected after parturition in two different seasons of winter and summer 2017. Cows were divided into 2 groups as normal cows with pH >5.8 or rumen pH ≤5.8. Ruminal fluid samples were collected through stomach tube for rumen pH and blood samples were taken from the coccygeal vein of cows concurrently once per day at days 4, 11 and 18 post-partum. SAA and Hp were determined in blood samples. The PROC MIXED of SAS (2003) was used for all determined variables with repeated measures. BCS, parity and milk yield were included as fixed and cows as random effect. The significant level was declared at P≤0.05, and tendency toward significance was considered at 0.05<P≤0.10 by the Tukey test. Correlation between rumen pH and APPs were surveyed using PROC CORRELATION of SAS (2003).
RESULTS:
The results of experiment showed that rumen pH was lower (P<0.05) in summer samples than winter (6.33 vs. 6.46). Rumen pH was lower (P<0.0001) in cows composed by subjects with rumen pH ≤5.8 than pH >5.8. For all examined cows, SAA concentration was greater in winter than summer (P<0.001), as well as at day 4 than days 11 and 18 after calving (P<0.05).Also, Hp concentration was greater for winter samples than summer (P<0.05), and at day 18 than days 4 and 11 after calving (333.33 vs. 299.3 and 300.1 respectively) (P<0.05). SAA and Hp concentrations were not affected by rumen pH. There was no significant correlation between rumen pH and APPs concentrations in both groups of pH ≤5.8 and pH >5.8.
CONCLUSIONS:
Results showed that rumen pH ≤5.8 seems not to stimulate the APPs production

Keywords

Article Title [Persian]

تغییرات pH شکمبه، غلظت خونی سرم آمیلوئید آ و هاپتوگلوبین در گاوهای شیرده هولشتاین تازه زا

Authors [Persian]

  • احمد پایداررود معجنی 1
  • صمد لطف اله زاده 1
  • غلامرضا قربانی 2
  • محمدرضا مخبر دزفولی 1
  • مرتضی موسوی 3
1 گروه بیماری های داخلی دام، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران
2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ایران
3 مدیر تولید شرکت دامپروری شریف آباد )مجموعه سرمایه گذاری اقتصادی کوثر(، قزوین، ایران
Abstract [Persian]

چکیده:
پیش زمینه: تشخیص شرایط التهابی مبتنی بر پروتئین های فاز حاد (APPs) در خون برای مدت طولانی در پزشکی انسانی مورد استفاده قرار گرفته است و اخیرا به عنوان یک ابزار تشخیصی در دامپزشکی مورد توجه می باشد.

اهداف: هدف از این مطالعه بررسی تغییرات مقدار برخی پروتئین های فاز حاد به عنوان پروتئین های فاز حاد اصلی در گاوهای شیری در روز اول پس از زایمان و ارتباط آنها با تغییرات PH شکم.

روش کار: د ر مجموع 106 راس گاو شیری تازه زا از نژاد هولشتاین به صورت تصادفی در دو فصل زمستان و تابستان 1395 به طور تصادفی انتخاب شدند. نمونه های مایع شکمبه از طریق لوله معده جمع آوری شد و نمونه های خون از ورید دمی به صورت همزمان در روزهای 4، 11 و 18 بعد از زایمان اخذ گردید. اندازه گیری pH شکمبه در مایعات شکمبه و SAA و Hp در نمونه های خون طی چند روز پس از زایمان انجام شد. تجزیه و تحلیل اطلاعات با استفاده از روش مختلط نرم افزار آماری SAS و همبستگی بین فراسنجه ها بررسی شد. نمره وضعیت بدنی، شکم زایش و تولید شیر شکم قبل به عنوان اثرات ثابت و گاو به عنوان اثر متغییر در مدل قرار گرفتند. اختلاف میانگین ها با آزمون چند دامنه ای توکی و سطح معنی داری 5% ≥ تعیین شد و بیش از 5 تا 10% به عنوان تمایل به معنی داری در نظر گرفته شد.

نتایج: نتایج آزمایش نشان داد در کل گاوهای مورد بررسی، مقدار pH مایع شکمبه در نمونه های تابستان (33/6) در مقایسه با زمستان (46/6) کمتر بود (05/0‌(P <.‌pH مایع شکمبه به طور معنی داری در گاوهای گروه 8/5 pH≤ (66/5) در مقایسه با گاوهای دارای pH طبیعی ( 47/6) کمتر بود (0001/0‌(P و Hp (08/0‌(P = بین گاوهای با pH شکمبه 8/5 و کمتر در مقایسه با گاوهای دارای pH شکمبه طبیعی تفاوت معنی دار نداشت. نتایج آزمایش نشان داد بین pH شکمبه و پروتئین های سرم آمیلوئید آ و هاپتوگلوبین همبستگی معنی داری وجود نداشت (05/0‌(P >. هیچگونه همبستگی معنی داری بین pH شکمبه و غلظت پروتین های فاز حاد در هر دو گروه pH ≤5.8 و pH> 5.8 وجود نداشت.

نتیجه گیری نهایی: تایج این مطالعه نشان داد که کاهش pH شکمبه در گاوهای شیری پس از زایمان با افزایش میزان SAA و Hp خون مرتبط نیست.

Keywords [Persian]

  • پروتئین فاز حاد
  • هاپتوگلوبین
  • سرم آمیلویدآ
  • گاو شیری
  • pH شکمبه

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Iranian Journal of Veterinary Medicine
Volume 14, Issue 1
January 2020
Pages 87-98

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