Effect of Hydro-Alcoholic Extract of Rosemary on Lipid Profile and Liver Enzymes in Male Wistar Rats Fed with High-Fat Diet

Articles in Press

Document Type : Pharmacology

Authors

1 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran

2 Department of Biology, Kazeroon Branch, Islamic Azad University, Kazeroon, Iran

3 Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran

Abstract

BACKGROUND: The liver plays a major role in the metabolic regulation of dietary nutrients. As a well-known risk factor, obesity is characterized by accumulating extra fat in the body. Previous studies have shown that high-fat diets result in non-alcoholic hepatic steatosis. The steatosis can lead to serious liver diseases. Because of the adverse effects of the available drugs for obesity, increasing efforts are being made to develop natural product-based safe and effective medicines.
OBJECTIVES: The present study aimed to assess the effects of hydroalcoholic extract of rosemary on lipid profile and liver enzymes in male Wistar rats fed with high-fat diet.
METHODS: The extract was prepared using an ethanol/distilled water (1:1) mixture. Thirty-two rats were randomly divided into four groups: control (standard diet), high-fat (HF) diet, RE (standard diet + rosemary extract), and HF+RE (HF diet + rosemary extract), and treated for 12 weeks. Biochemical parameters were determined using standard methods.
RESULTS: The total cholesterol changed from 54.2 mg/dL in control group to 76.8 mg/dL in HF diet. In the RE group, the high-density lipoprotein (HDL) and low-density lipoprotein (LDL) enhanced and decreased, respectively, compared to the standard regimen. The extract caused 14.4% reduction (p<0.05) in triglyceride levels in comparison to the HF rats. Compared to the HF diet, the HF+RE diet caused significant (p<0.05) reduction in the aspartate transaminase (AST) and alanine transaminase (ALT).
CONCLUSIONS: Although hydroalcoholic extract of rosemary was relatively effective to diminish the influence of high‒fat diet on total cholesterol and liver enzymes, to evaluate the recognized safety and efficacy of the extract, more investigations must be performed.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 01 May 2022

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