Document Type : Original Articles
Authors
1
Department of Veterinary Physiology and Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria
2
Department of Veterinary Pharmacology and Toxicology, Federal University of Agriculture, Abeokuta, Nigeria
3
Veterinary Teaching Hospital, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
10.22059/ijvm.2023.358146.1005389
Abstract
Diabetes mellitus (DM) is a metabolic illness, defined by elevated level of blood sugar because of a problem with insulin synthesis, action, or both. Various clinical signs follow DM, majorly hyperglycaemia, polydipsia, polyuria, and polyphagia. Worldwide prevalence is high and predicted to rise to 592 million by 2035. Animal models are used in the study of diabetes due to ethical issues. Streptozotocin (STZ) model is frequently used but has poor dependability due to unexplained acute toxicity and effective dose variability. This research was conducted to determine the effective dose regimen of STZ for inducing diabetes in Wister rats.
28 male Wistar rats (160 - 190 g) were randomly divided into 4 groups (n=7) and monitored for 21 days after diabetes induction with STZ: Control (CTR), diabetics: DIA1 (60 mg/kg STZ), DIA2 (60 mg/kg STZ twice at 0 and 24 hours), and DIA3 (60 mg/kg STZ thrice at 0, 24 and 48 hours). Plasma glucose was determined with a glucometer. Body weights, feed intake, and faecal output were weighed with a digital balance, while water intake and urine output were measured with a measuring cylinder. Analyses of data obtained were performed using a One-way ANOVA and Tukey’s test at p<0.05 for significance.
There was significant (p<0.05) decrease in body weight of the diabetics (-15.53±1.2, -26.8±1.2, -28.5±1.9%) compared to the CTR (10.5±2.5%). There was significant (p<0.05) increase in fasting blood glucose concentrations (135.2±9.0, 273.2±6.5, 257.0±5.3 mg/dL) in the diabetics compared to the CTR (79.3±1.1 mg/dL). Water intake (56.9±0.9, 72.1±1.7, 77.8±5.5 mL), feed intake (19.4±0.6, 23.3±1.9, 42.1±2.1 g), voided urine (6.34±0.1, 8.39±0.88, 9.58±0.50 mL) and voided faeces (10.4±0.26, 11.7±0.43, 8.5±0.17 g) in the diabetics increased significantly (p<0.05) compared to the CTR (26.5 ± 0.8 mL, 13.4±0.3 g, 1.84±0.08 mL, and 6.5±0.33 g respectively).
The dose regimen of 60 mg/kg STZ administered intraperitoneally twice (24 hours apart) sustained diabetes for 21 days. We recommend that this dose regimen be adopted in STZ-induced diabetic studies in male Wistar rats.
Keywords
Article Title [Persian]
رژیم دوز موثر STZ برای دیابت ناشی از STZ در مدل موش صحرایی
Authors [Persian]
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اولوشولا آدلیه
1
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تمی توپه آجالا
1
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اولوواتودیمو ادووله آدکویا
2
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آدنیک لیابو آدلیه
3
1
گروه فیزیولوژی دامپزشکی و بیوشیمی، دانشگاه فدرال کشاورزی، آبئوکوتا، نیجریه
2
گروه فارماکولوژی دامپزشکی و سم شناسی، دانشگاه فدرال کشاورزی،
آبئوکوتا، نیجریه
3
بیمارستان آموزشی دامپزشکی، دانشگاه فدرال کشاورزی، آبئوکوتا، ایالت اوگون، نیجریه.
Keywords [Persian]
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رژیم دوز
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دیابت
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استرپتوزوتوسین
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موش صحرایی ویستار
)