Document Type : Original Articles
Authors
1
HIPRA Laboratorios S.A., Av. Selva, Amer, Spain Department of Pathology and Veterinary Public Health, Avian Pathology Unit, IAV Hassan II, , Rabat, Morocco
2
Department of Pathology and Veterinary Public Health, Avian Pathology Unit, IAV Hassan II, , Rabat, Morocco
3
HIPRA Laboratorios S.A., Av. Selva, Amer, Spain
4
Department of Pathology and Veterinary Public Health, Microbiology, Immunology and Infectious Diseases Unit, IAV Hassan II, , Rabat, Morocco
5
Epidemiology and Veterinary Microbiology Laboratory, Institute Pasteur of Tunis, University Tunis El Manar, Tunisia
6
Mouahid’s Private Vet Clinic, Bd. Hassan I Lot 20 Appt 1 Hay Abbadi, Temara, Morocco
7
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra,Ozzano dell’Emilia
10.22059/ijvm.2023.359991.1005404
Abstract
Background. Avian Metapneumovirus (aMPV) has been proven to be a widespread infectious respiratory pathogen affecting turkeys and chickens, with co-predominance of the subtypes A and B.
Objectives. No official reports exist in Morocco about the subtypes of aMPV circulating. Hence, using quantitative Reverse-Transcriptase Polymerase Chain Reaction (qRT-PCR) subtypes-specific A and B, we aimed at detecting and identifying the potential subtype(s) circulating.
Methods. We conducted a longitudinal study on three broiler flocks strictly not vaccinated against aMPV and located in two different geographical regions and two flocks that expressed typical swollen head syndrome (SHS) and sampled once. Furthermore, we sampled dead birds of one flock confirmed seropositive from a previous study. 118 swabs pooled in 24 samples were subjected to the ribonucleic acid (RNA) extraction and amplified using a triplex RT-PCR for specific detection of aMPV subtypes A and B.
Additionally, serum samples were taken at slaughtering age to cross-check the molecular results. A total of 84 sera were analyzed with a commercial indirect Enzyme-Linked Immunosorbent Assay (ELISA) kit to detect and titer antibodies against the two subtypes.
Results. Avian Metapneumovirus was detected by qRT-PCR in all the flocks. 87.50% of the samples were positive for aMPV-B, and 16.67% for aMPV-A and aMPV-B simultaneously. All the flocks showed seropositivity, confirming the molecular findings.
Conclusion. The present investigation is the first molecular study in Morocco to elucidate the circulation of aMPV-A and aMPV-B in broiler farms in Morocco with a dominance of aMPV-B and the possibility of co-presence of both subtypes.
Keywords
Article Title [Persian]
تشخیص متاپنوموویروس پرندگان زیرگروه A و B در مزارع مرغ گوشتی مراکش
Authors [Persian]
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امینه مرنیزی
1
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اومیمه کدیری
2
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جوان لویس کریادو
3
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محمد بوسلیخانه
4
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عبدالجلیل گرام
5
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محمد موحید
6
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النا کاتلی
7
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سدیا ناسیک
2
1
آزمایشگاهای هیپرا. سلوا، عامر، اسپانیا
گروه پاتولوژی و بهداشت عمومی دامپزشکی، واحد آسیب شناسی پرندگان،
رباط، مراکش
2
گروه پاتولوژی و بهداشت عمومی دامپزشکی، واحد آسیب شناسی پرندگان،
رباط، مراکش
3
آزمایشگاهای هیپرا. سلوا، عامر، اسپانیا
4
گروه پاتولوژی و بهداشت عمومی دامپزشکی، میکروبیولوژی، ایمونولوژی و
واحد بیماری های عفونی، رباط، مراکش
5
آزمایشگاه اپیدمیولوژی و میکروبیولوژی دامپزشکی، انستیتو پاستور تونس، دانشگاه تونس ،ال منار، تونس
6
کلینیک خصوصی دامپزشکی موحید، عبادی، تمارا، مراکش
7
گروه علوم پزشکی دامپزشکی، دانشگاه بولونیا،بولونیا ،ایتالیا
Keywords [Persian]
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متاپنوموویروس پرندگان؛ جوجه های گوشتی
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مراکش؛ RT-PCR
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ساب تایپ
)