Total Anthocyanin Extract from Purple Sweet Potato Improves Neurotransmitter and Locomotor Behaviour by Altering Brain Corticosterone in Chronic-Stressed Mice

Document Type : Original Articles

Authors

1 Department of Physiology, Faculty of Medicine, Universitas Brawijaya, ،Malang, Indonesia- Research Centre of Smart Molecule of Natural Genetics Resources, Universitas Brawijaya, Malang, Indonesia

2 Bachelor Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

3 Department of Physiology, Faculty of Medicine, Universitas Brawijaya, ،Malang, Indonesia

10.22059/ijvm.2023.360230.1005405

Abstract

Objectives: Prolonged stress has essential roles on depression disorder through brain inflammation and neurotransmitter imbalances. Natural plant antioxidant is promising to resist the negative impact of stress. This study purposed to analyse the effect of total anthocyanin extracts (ANC) from purple sweet potatoes on brain inflammation, neurotransmitters, and locomotor behaviour of chronic-stressed mice model Methods: Twenty males of adult BALB/c mice were assigned to control; stress; stress + ANC 10 mg/kgBW; stress + ANC 20 mg/kgBW and stress + ANC 40 mg/kgBW. Restraint stress was applicated 2 hours/day for 14 days. Enzyme-linked immunosorbent assay (ELISA) was performed to measure brain corticosterone, dopamine, and gamma-aminobutyric acid (GABA) level. Locomotor behaviour was analysed using open field test at pre- and post-ANC treatment. Molecular docking prediction was performed between ANC and monoamine oxidase-B (MAO-B) enzyme. Results: Administration of ANC decreased brain corticosterone level. Neurotransmitter of dopamine decreased in stress-induced group then increased following ANC treatment. Increased of GABA level observed in stressed and treated groups. Locomotor analysis showed tendencies of reduction of total distance movement and velocity after ANC treatment. Molecular prediction showed ANC has potential to inhibit the MAO-B enzymes. Conclusion: The ANC from PSP relieved brain inflammation and modified the neurotransmitter of dopamine and GABA thus affect the locomotor of chronic stressed-induced mice. Further in vivo studies are necessary to evaluate the molecular mechanism of ANC from PSP in chronic stress exposure particularly on MAO enzyme regulation.

Keywords

Article Title [Persian]

عصاره آنتوسیانین از سیب زمینی شیرین بنفش با تغییر کورتیکوسترون مغز در موش های تحت استرس مزمن، انتقال دهنده های عصبی و رفتار حرکتی را بهبود می بخشد.

Authors [Persian]

  • نیا کورنیانینگ سی 1
  • آرلیا رامادینی حکیم 2
  • دافا سال سابیلا 2
  • آگوین فهمی فهانانی 3
  • نویتا تیتیس هربیانتی 3
  • رتی راتناواتی 3
1 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه براویجایا، مالانگ ،اندونزی- مرکز تحقیقات مولکول هوشمند منابع ژنتیک طبیعی، دانشگاه براویجایا، مالانگ، اندونزی
2 برنامه لیسانس علوم پزشکی، دانشکده پزشکی، دانشگاه براویجایا،مالانگ، اندونزی
3 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه براویجایا، مالانگ ،اندونزی

Keywords [Persian]

  • آنتوسیانین
  • مغز
  • دوپامین
  • گابا
  • استرس
Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 09 July 2023

Files

Share

How to cite

Statistics