The Chemical Composition and Antibacterial Effect of Essential Oils of Rosemary and Basil in Milk

Articles in Press

Document Type : Original Articles

Authors

1 Department of Animal Science, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran

2 M.Sc. graduate of Department of Animal Science, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran

3 Biology Department, Faculty of Science, University of Gonbad Kavous, Gonbad Kavous, Iran .

Abstract

Background: According to the diverse side effects of antibiotics, new and natural antibacterial substances are needed to treat bacterial diseases and one of these substances is the essential oils (EOs) of medicinal plants. Milk fat and protein may reduce the antimicrobial impact of plant EOs. Objectives: This study investigated the antibacterial activity of rosemary and basil EOs in comparison with lincospectinomycin antibiotic on three mastitis causing-bacteria including Streptococcus agalactiae, Staphylococcus aureus, and Escherichia coli bacteria in the milk media.
Methods: Chemical compounds of EOs were identified by gas chromatography. The minimum bactericide concentration (MBC) and the minimum inhibitory concentration) MIC) of essential oils were studied by the tube dilution method and the growth curve of bacteria was studied at 0, 6, 10, and 24 hours.
Results: The most important compounds of rosemary were carene (45.11%) and eucalyptus (20.62%), and those of basil were estragol (70.42%) and carene (17.99%). MIC and MBC of rosemary were lower than lincospectinomycin and those of basil were the same as lincospectinomycin. At 6-h, the bacterial reduction of E. coli and S. agalactiae bacteria was significant and population reduction of rosemary was significant for S. aureus. At 24 h, rosemary and basil significantly diminished the bacterial count of S. aureus, as well as, basil significantly decreased the S. agalactiae.
Conclusion: In general, the antibacterial effect of the EOs was acceptable, and clinical studies are recommended for the treatment of other diseases, including mastitis.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 27 May 2024

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