Document Type : Original Articles
Authors
1
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Kufa, Najaf, Iraq Department of Department Anesthesia, The Islamic University, Najaf, Iraq.
2
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Kufa, Najaf, Iraq
Abstract
BACKGROUND The study took place at the animal facility of the University of Kufa College of Veterinary Medicine. The primary objective was to examine the impact of propolis (Pro) and Vitamin E on the reproductive hormones (follicle stimulating hormone FSH, Luteinizing hormone LH and Testosterone T), histopathological changes, and gene expression of 3β-HSD1mRNA in male rats with induced testicular dysfunction by Bisphenol A(BPA).
OBJECTIVE The primary objective of the current study was to examine the toxic effects of BPA.on the reproductive organs and roles propolis on the regulation of Pituitary- Gonad Axis and Gene Expression of adult male rats, and comparation with vitamin E. which focused on evaluated, male reproductive hormones (Follicle Stimulating Hormone FSH, Luteinizing Hormone LH and Testosterone T) histopathological changes, and gene expression of 3β-HSD1mRNA.
MATERIAL AND METHODS The rats were divided into five groups: Group 1 received standard food and water (negative control), Group 2 received 0.2 mL of corn oil (vehicle control), Group 3 received BPA at 50 mg/kg body weight, Group 4 received Propolis at 250 mg/kg body weight followed by BPA, and Group 5 received vitamin E at 100 mg/kg body weight followed by BPA. BPA was administered via intraperitoneal injection three days a week for three weeks, while Propolis and vitamin E were given orally.
RESULTS the result showed the Bisphenol A was significant adverse effects male reproductive hormones (FSH, LH and T) histopathological changes, and gene expression of 3β-HSD1 mRNA. In contrast, the propolis and vitamin E groups showed was positively influenced on all parameters.
Conclusion: Bisphenol A exposure induced histopathological changes and male reproductive hormones (FSH, LH and T) of male rats and gene expression of 3β-HSD1 mRNA of (Testosterone). Propolis and Vitamin E was positively influenced on histopathological changes and male reproductive hormones (FSH, LH and T) induced by Bisphenol A or restored their normal architecture.
Keywords
Article Title [Persian]
بررسی نقش بره موم و ویتامین E بر محور هیپوفیز-گناد و بیان ژن هورمون تستوسترون در موش‌های صحرایی نر مسمومیت بیضه
Authors [Persian]
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علی مان مظفر
1
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حمزه فواد زیدان
2
1
گروه فیزیولوژی، بیوشیمی و فارماکولوژی، دانشکده دامپزشکی، دانشگاه کوفه، نجف، عراق
گروه بیهوشی، دانشگاه اسلامی، نجف، عراق.
2
گروه فیزیولوژی، بیوشیمی و فارماکولوژی، دانشکده دامپزشکی، دانشگاه کوفه، نجف، عراق
Keywords [Persian]
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3β-هیدروکسی استروئید دهیدروژناز نوع 1 (3β-HSD1)
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اسپرم
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بیضه
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آسیب بافت شناسی
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تشخیص جنسی
)