Document Type : Original Articles
Authors
Department of Physiology, Biochemistry, and Pharmacology, Faculty of Veterinary Medicine University of Kufa, Najaf, Iraq
10.22059/ijvm.2024.374252.1005540
Abstract
OBJECTIVE: This study aims to investigate.The protective of eugenol against nephrotoxicity induced by iron overload in male rats.
METHODS: A total of 30 rats were randomly divided into six equal groups as follows: the 1st group controlled negative C- I.P injection of distilled water. The 2nd group, control positive C+, received iron dextran only at 100 mg/kg-BW I.P. The 3rd and 4th groups (IE1& IE2, respectively) received iron dextran 100 mg/kg-BW I.P and eugenol 50,100 mg/kg-BW orally, respectively, simultaneously. The 5th and 6th groups (E3& E4 respectively) received eugenol only at 50,100 mg/kg-BW orally.
RESULTS: The study's findings revealed significant improvements in various biomarkers and histological characteristics in rats treated with eugenol compared to the control group (C+). Specifically, rats treated with eugenol exhibited decreased levels of creatinine, blood urea nitrogen (BUN), Malondialdehyde (MDA), Erythropoietin (EPO), and kidney injury molecule-1 (Kim-1), along with increased concentrations of Glutathione (GSH). The microscopical examination of C-, E3, and E4 treated groups showed typical histological architecture of the kidney. However, the C+ showed epithelial cell necrosis for renal tubules and inflammatory processes in the renal section, especially glomeruli and interstitial section of proximal renal tubules. The IE1 showed moderate necrosis of epithelial cells of renal tubules and inflammation of the renal section. On the other hand, IE2 showed Normal histological architecture of the cortex and a mild inflammatory process in the medulla.
CONCLUSION: The present study, in conclusion, eugenol has an ameliorative effect against iron overload-induced nephrotoxicity in male rats.
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