Document Type : Original Articles
Authors
1
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
2
Immunity Unit, Agriculture Research Center (ARC), Animal Reproduction Research Institute, Giza, Egypt.
3
Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
4
Department of Artificial Insemination and Embryo Transfer, Agriculture Research Center (ARC), Animal Reproduction Research Institute, Giza, Egypt.
10.32598/ijvm.19.2.1005551
Abstract
Background: Antioxidant supplementation promotes the fertilizing capacity of post-thawed ram spermatozoa.
Objectives: This study was designed to validate the effect of different levels of coumestrol as an antioxidant on post-thawing parameters of cryopreserved spermatozoa in rams.
Methods: A total of 60 ejaculates were collected from six sexually mature Barki rams. The accepted semen samples were extended, pooled, cooled, and then divided into five aliquots: A control group (tris-based egg yolk extender) without coumestrol addition, and 4 aliquots with concentrations of 0.1, 1, 10, and 100 µM of coumestrol. These aliquots were then subjected to the cryopreservation process. The control and treated frozen semen were thawed and assessed for motility using computer-assisted sperm analysis (CASA), total antioxidant capacity (TAC), malondialdehyde (MDA) levels, mitochondrial activity, comet assay for DNA integrity, acrosome integrity, and casein kinase 2 alpha 2 (CK2α2) gene expression.
Results: The addition of 1 µM of coumestrol significantly improved progressive motility. Metabolic activity was significantly higher in semen treated with 1, 10, and 100 µM coumestrol compared to control and 0.1 µM coumestrol groups. Furthermore, acrosome integrity was significantly higher in the 0.1 and 1 µM of coumestrol groups. In the 1, 10, and 100 µM coumestrol groups, TAC was significantly higher than in controls. Furthermore, MDA levels were significantly lower in all coumestrol groups compared to the controls. The comet assay exhibited a significant reduction in fragmented DNA in semen treated with coumestrol, especially with the addition of 1 µM coumestrol. The expression of CK2α2 showed a significant fold decline in semen supplemented with 10 and 100 µM coumestrol compared to the control group.
Conclusion: The addition of 1 µM of coumestrol could ameliorate the deleterious impacts of cryo-damage by improving the sperm antioxidant capacity, mitochondrial activity, and both acrosome and DNA integrity.
Keywords
Article Title [Persian]
تأثیر مکمل کومسترول بر انجماد مایع منی گوسفند
Authors [Persian]
-
دعا حسنی السعید
1
-
لیلا اسماعیل کورتم
2
-
هبا محمد احمد عبدلرازق
3
-
احمد منیر
4
1
گروه مامایی، دانشکده دامپزشکی، دانشگاه کانال سوئز، اسماعیلیه،
مصر
2
بخش ایمنی شناسی، موسسه تحقیقات تولید مثل حیوانات، مرکز تحقیقات کشاورزی (ARC)، جیزه،
مصر
3
گروه فیزیولوژی، دانشکده دامپزشکی، دانشگاه کانال سوئز، اسماعیلیه، مصر
4
گروه تلقیح مصنوعی و انتقال جنین، پژوهشکده تکثیر دام،
مرکز تحقیقات کشاورزی (ARC)، جیزه، مصر.
Keywords [Persian]
-
بیان کازئین کیناز 2
-
کومسترول
-
انجماد
-
فعالیت میتوکندری
-
مایع منی قوچ
)