Document Type : Original Articles
Authors
1
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
2
Immunity Unit, Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt.
3
Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
4
Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt.
Abstract
Background: Cryopreservation extender supplementation with antioxidants promotes the fertilizing capacity of the post-thawed spermatozoa.
Objectives: the study was designed to validate the effect of different levels of coumestrol on post-thawing functions of cryopreserved ram spermatozoa.
Methods: A total of 60 ejaculates were collected from 6 sexually mature Barki rams. The accepted semen samples were extended, pooled, cooled, divided into 5 aliquots: control (tris-based egg yolk extender), 0.1, 1, 10 and 100 µM of coumestrol and undergone cryopreservation process. Frozen semen groups were thawed and assessed for motility with CASA, total antioxidant capacity (TAC), malondialdehyde level (MDA), mitochondrial activity, comet assessed DNA integrity, acrosome integrity and casein kinase 2 alpha 2 (CK2α2) expression.
Results: Addition of coumestrol 1µM significantly improved progressive motility. Metabolic activity was significantly higher 1, 10 and 100 µM compared to control and 0.1µM coumestrol. Furthermore, acrosome integrity was significantly higher in 0.1 and 1µM of coumestrol groups. In 1, 10 and 100µM coumestrol groups TAC were significantly higher than control. Furthermore, MDA levels were significantly lower in all coumestrol groups when compared to control. Comet assay exhibited significant reduction in fragmented DNA for all coumestrol groups, especially with addition of 1 µM coumestrol. The expression of CK2α2 showed a significant fold decline with supplementation of 10 and 100 µM coumestrol compared to control group.
Conclusion: the addition of 1 µM coumestrol could ameliorate the deleterious impacts of cryo-damage via improving the sperm antioxidant capacity, mitochondrial activity and both acrosome a well as DNA integrities.
Keywords
Article Title [Persian]
تأثیر مکمل کومسترول بر انجماد مایع منی گوسفند
Authors [Persian]
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دعا حسنی السعید
1
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لیلا اسماعیل کورتم
2
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هبا محمد احمد عبدلرازق
3
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احمد منیر
4
1
گروه مامایی، دانشکده دامپزشکی، دانشگاه کانال سوئز، اسماعیلیه،
مصر
2
بخش ایمنی شناسی، موسسه تحقیقات تولید مثل حیوانات، مرکز تحقیقات کشاورزی (ARC)، جیزه،
مصر
3
گروه فیزیولوژی، دانشکده دامپزشکی، دانشگاه کانال سوئز، اسماعیلیه، مصر
4
گروه تلقیح مصنوعی و انتقال جنین، پژوهشکده تکثیر دام،
مرکز تحقیقات کشاورزی (ARC)، جیزه، مصر.
Keywords [Persian]
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بیان کازئین کیناز 2
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کومسترول
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انجماد
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فعالیت میتوکندری
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مایع منی قوچ
)