Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c

Articles in Press

Document Type : Original Articles

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran

3 Department of Embryology, Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran.

Abstract

BACKGROUND: One of the most important aspects of artificial insemination in animals is the practical design of long-term preservation of sperm. Although there are many reports on canine sperm storage methods, but an improved system for chilling of canine spermatozoa is required for successful breeding programs in companion and working dog colonies.

OBJECTIVES: The aim of this study was to compare the effects of Bain-marie thermal shock on dog sperm parameters before cooling.

METHODS: Ejaclated sperm from testes of mature healthy dogs in veterinary center were collected and divided into two groups: C:TRIS+ egg yolk 20% (control, without incubation at 37°C), T: TRIS+ egg yolk 20% (treatment group, incubation at 37°C for 10 minutes). Evaluation of cooled Sperm was done by CASA for motility test, morphology, Eosin- Nigrosin vital staining and Hypo-Osmotic Swelling Test (HOST) after cooling.

RESULTS: In heat shock samples, total and progressive motility, VCL, VSL, VAP, LIN, STR, the proportion of sperm with normal morphology, the proportion of viable sperm and the proportion of sperm positive for HOST were not affected by the interaction of group by time. However, they were lower in treatment than control group (P < 0.05) and they decreased over the course of preservation (P < 0.001).

CONCLUSIONS: Keeping sperm at a temperature of 37°C and applying heat shock by Bain-Marie has extremely negative effects on the quality of ejaculated sperm.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 11 September 2024

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