Physicochemical parameters affecting the invitro toxins production by characterized antibiotic-resistant Clostridium perfringens toxinotype B Isolates

Articles in Press

Document Type : Original Articles

Authors

1 Institute of Microbiology, Faculty of Veterinary science, University of veterinary and animal sciences, Lahore Pakistan

2 Institute of Microbiology, Faculty of veterinary science, university of veterinary and animal sciences, Lahore, Pakistan

3 Institute of Biochemistry and Biotechnology, faculty of Bioscience, university of veterinary and animal sciences, Lahore, Pakistan.

4 Institute of Microbiology, Faculty of Veterinary science, University of veterinary and animal sciences, Lahore, Pakistan

Abstract

Background: Alpha, beta and epsilon toxins of Clostridium perfringens toxinotype B are the major potent toxins involved in pulpy kidney disease. To combat it, proper vaccination of animals is required with an effective vaccine.
Objective: The research study was planned to document the effect of Syzygium cumini extract on resistant bacteria and in-vitro toxin production potential of Clostridium perfringens toxinotype B under the influence of various physicochemical parameter.
Materials and method: C. perfringens isolates were characterized on the basis of biochemical tests, toxinotyping and 16S rRNA typing. C. perfringens toxinotype B indigenously characterized isolates were subjected to antibiotic susceptibility profiling through Kirby-Bauer method. Against resistant isolates (n=06), S. cumini leaves ethanolic extract’s antibacterial activity was evaluated through well diffusion assay and minimum inhibitory concentration (MIC) was determined. C. perfringens type B isolates subjected to toxins production optimization under physicochemical parameter.
Results: Isolates were identified as toxinotype B. Maximum zone of inhibition (ZOI) of 21.63±0.80 mm and MIC value of 7.18±5.13mg/mL was recorded for S. cumini leaves ethanolic extract. Isolates MW551887.1 and MW332247.1 produced higher hemolytic and cytotoxic units of toxins (alpha, beta and epsilon) at 0.2% glucose concentration after 24 hours at 37ºC, respectively.
Conclusion: These isolates could be used at industrial level for higher toxins production under optimized physicochemical parameters for vaccine production. Alpha, beta and epsilon toxins of C. perfringens toxinotype B are involved in causing pulpy kidney disease. To combat disease, control of antibiotic resistance and proper vaccination of animals is crucial.

Keywords

Article Title [Persian]

پارامترهای فیزیکوشیمیایی مؤثر بر تولید توکسین‌های آزمایشگاهی توسط جدایه‌های کلستریدیوم پرفرنجنس توکسینوتیپ B مقاوم به آنتی‌بیوتیک

Authors [Persian]

  • مدیحه طارق 1
  • آفتاب انجم 2
  • وجیها طارق 3
  • تیحریم علی 4
  • رابعه منظور 2
1 موسسه میکروبیولوژی، دانشکده علوم دامپزشکی، دانشگاه دامپزشکی و علوم دامی، لاهور پاکستان
2 موسسه میکروبیولوژی، دانشکده علوم دامپزشکی، دانشگاه دامپزشکی و علوم دامی، لاهور پاکستان
3 موسسه بیوشیمی و بیوتکنولوژی، دانشکده علوم زیستی، دانشگاه دامپزشکی و علوم دامی، لاهور، پاکستان.
4 موسسه میکروبیولوژی، دانشکده علوم دامپزشکی، دانشگاه دامپزشکی و علوم دامی، لاهور، پاکستان

Keywords [Persian]

  • مقاوم به آنتی بیوتیک؛ سم آلفا؛ سم بتا؛ کلستریدیوم پرفرنجنس توکسینوتیپ B
  • سم اپسیلون؛ گلوکز
Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 06 October 2024

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