Physicochemical parameters affecting the invitro toxins production by characterized antibiotic-resistant Clostridium perfringens toxinotype B Isolates

Articles in Press

Document Type : Original Articles

Authors

1 Institute of Microbiology, Faculty of Veterinary science, University of veterinary and animal sciences, Lahore Pakistan

2 Institute of Microbiology, Faculty of veterinary science, university of veterinary and animal sciences, Lahore, Pakistan

3 Institute of Biochemistry and Biotechnology, faculty of Bioscience, university of veterinary and animal sciences, Lahore, Pakistan.

4 Institute of Microbiology, Faculty of Veterinary science, University of veterinary and animal sciences, Lahore, Pakistan

Abstract

Background: Alpha, beta and epsilon toxins of Clostridium perfringens toxinotype B are the major potent toxins involved in pulpy kidney disease. To combat it, proper vaccination of animals is required with an effective vaccine.
Objective: The research study was planned to document the effect of Syzygium cumini extract on resistant bacteria and in-vitro toxin production potential of Clostridium perfringens toxinotype B under the influence of various physicochemical parameter.
Materials and method: C. perfringens isolates were characterized on the basis of biochemical tests, toxinotyping and 16S rRNA typing. C. perfringens toxinotype B indigenously characterized isolates were subjected to antibiotic susceptibility profiling through Kirby-Bauer method. Against resistant isolates (n=06), S. cumini leaves ethanolic extract’s antibacterial activity was evaluated through well diffusion assay and minimum inhibitory concentration (MIC) was determined. C. perfringens type B isolates subjected to toxins production optimization under physicochemical parameter.
Results: Isolates were identified as toxinotype B. Maximum zone of inhibition (ZOI) of 21.63±0.80 mm and MIC value of 7.18±5.13mg/mL was recorded for S. cumini leaves ethanolic extract. Isolates MW551887.1 and MW332247.1 produced higher hemolytic and cytotoxic units of toxins (alpha, beta and epsilon) at 0.2% glucose concentration after 24 hours at 37ºC, respectively.
Conclusion: These isolates could be used at industrial level for higher toxins production under optimized physicochemical parameters for vaccine production. Alpha, beta and epsilon toxins of C. perfringens toxinotype B are involved in causing pulpy kidney disease. To combat disease, control of antibiotic resistance and proper vaccination of animals is crucial.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 06 October 2024

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