Comparison the Efficiency of Three Staining Methods Including Giemsa, Leishman and Leishman-Giemsa for Evaluation Fish Blood Smear

Articles in Press

Document Type : Original Articles

Authors

1 Graduated from the Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.

2 Department of Aquatic Animal Health and Diseases, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

Abstract

Backgrounds: The haematological panel provides valuable information about the physiologic status and health of the fish in the aquatic expert's test through the optimal staining of the nucleus, cytoplasm, and cytoplasmic granules.
Objectives: Our research focused on creating a new Leishman-Giemsa dye mixture specifically for fish blood smears and evaluating its effectiveness compared to standard staining techniques.

Methods: Blood samples were taken from 20 healthy common carp to produce three groups of peripheral blood smears: one for Leishman-Giemsa dye and two for Leishman's and Giemsa's stains. Two experienced clinical pathologists extensively examined all three types of blood smears based on four staining characteristics: nuclear characteristics of erythrocytes and leukocytes, cytoplasmic characteristics, and leukocyte granulation. Two experts' mean ratings for each staining technique were compared.
Results: Regarding RBC and WBC nuclei characteristics, the two conventional Leishman and Giemsa staining methods yielded substantially lower mean scores than the new Leishman-Giemsa staining method (P<0.05). Leishman-Giemsa staining enhanced the clarity of RBC and WBC nuclear characteristics. The new Leishman-Giemsa staining technique resulted in a statistically significant (P<0.05) difference in the cytoplasmic characteristics of fish WBC compared to the other two methods.
Conclusion: This study's results demonstrated for the first time that fish blood cells stained with the novel Leishman-Giemsa method are more desirable. In addition, its nuclear and cytoplasmic staining is superior to conventional Giemsa and Leishman stains when used separately. The Leishman-Giemsa cocktail has a high index for air-dried smear discolouration.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 06 October 2024

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