The Impact of Cytopathic and Non-Cytopathic Biotypes of Bovine Viral Diarrhea Virus on Total Antioxidant Capacity of Bovine Oocytes In-Vitro

Articles in Press

Document Type : Original Articles

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Background
Bovine Viral Diarrhea Virus (BVDV) is the most significant pathogen in dairy cattle herds.
Objectives
The objective of this research was to examine the impact of cytopathic (CP) and non-cytopathic (NCP) biotypes of BVDV on the total antioxidant capacity of bovine oocytes in vitro.
Materials & Methods
Oocytes were obtained from slaughtered bovine ovaries, washed, and matured in maturation medium. The oocytes were divided into five distinct groups, each consisting of at least 60 oocytes. The control group was not exposed to any BVDV biotypes. Oocytes were challenged with cytopathic (CP) and non-cythopatic (NCP) BVDV at two distinct concentrations of 104 and 105 tissue culture infectious doses (TCID) 50/mL. To determine the antioxidant capacity of oocytes, a Total Antioxidant Capacity Assay was conducted after two hours of incubation. Graph Pad Prism 8.4.3 was utilized to analyze the data. A one-way ANOVA was performed, accompanied by post-hoc Tukey's HSD test.
Results
Results indicated that only the CP biotype of BVDV significantly decreased the total antioxidant capacity of infected oocytes when compared to the control group whereas the NCP biotype did not significantly alter the total antioxidant of infected groups.
Conclusion
Our study showed that only CP BVDV in 104 and 105 (TCID)50/mL doses had an effect on the infected oocytes and decreased the oocyte's total antioxidant capacity significantly while the NCP biotype did not significantly alter the infected oocyte's total antioxidant capacity.

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 06 October 2024

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