In Vivo Antitrypanosomal Activities of Methanolic Extract of Lawsonia inermis Linn. Leaves on Trypanosome Brucei Infected Wistar rat

Articles in Press

Document Type : Original Articles

Authors

1 Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.

2 Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.

3 Department of Veterinary Physiology and Biochemistry Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria..

4 Department of Veterinary Microbiology Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria.

Abstract

Background: Trypanosomiasis is a major problem affecting both human and animals. Nearly 30,000 personalities in various nations of Sub-Saharan Africa have African trypanosomiasis. It has led to about 21,000 deaths yearly.
Objectives: This experiment was conducted to evaluate the anti-trypanocidal effects of Lawsonia inermis in rats infected with T. brucei.
Methods: 30 rats were allotted to groups (1-5) of six rats each. Group 1 (negative control), 2 (tryps control), 3 (Diminazene (DA) 7 mg/kg), 4 (Lawsonia inermis (LI) at 200 mg/kg), and 5 (DA+LI). All rats in groups (2-5) were infected with 3x106 of Trypanosoma brucei per ml of blood.
Result: Percentage weight gain of rats in diminazene extract showed increased weight gain (6.3%) when compared to tryps-control. Diminazene showed significant weight gain when compared with the negative control. The survivability rate showed that DA, LI and DA+LI combination survived for 14 days without visible relapse. The PVC, RBC, WBC, platelets and MCV increased significantly in the extract treated groups while MCH and MCHC decreased significantly. Lymphocytes, Monocytes, Eosinophils and Basophils increased significantly compared to the control. Globulin, AST, ALT, ALP and Blood urea nitrogen increased non-significantly. Creatinine and Total bilirubin significantly decreased compared to untreated control. Lawsonia inermis significantly increased GSH, GST, GPx, SOD, while decreasing MDA and inflammatory cytokines (IL-1, 6, and 12) when compared to untreated control groups.
Conclusions: Lawsonia inermis reduced the level of parasitemia in a transient phase and drug-extract combination cleared the parasitemia within shortest time

Keywords

Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 24 December 2024

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