To Try to Understand the Dynamic Process of Rat Ovary through Histological Sections and Cell Culture Studies

Document Type : Original Articles

Authors

1 Department of Histology and Embryology, Faculty of Medicine, Pamukkale University, Denizli, Turkey

2 Department of Histology and Embryology, Faculty of Medicine, Pamukkale University, Denizli, Turkey- Young Researchers and Elite Club, Borujerd Branch, Islamic Azad University, Borujerd

10.22059/ijvm.2024.367617.1005464

Abstract

Objectives: To examine the development of cells and structures in the ovary both in cell culture medium and in histological sections, and to observe the effects of the niche formed by them on folliculogenesis.
Methods: We had 3 different groups in our study, and each group included 3 rats. The ovaries of three prepubertal female rats were taken under sterile conditions for cell culture. To prepare histological sections, ovarian tissues of early breeding period rats and late breeding period rats were excised and passed through routine histological tissue process steps.
Result: Ovarian surface epithelium and stromal cells were observed to proliferate in cell culture. Primordial follicle-like structures were observed between the surface epithelial cells. Histological examination revealed periovary adipose tissue around the ovary, separated from the surface epithelium by a thin fibrous sheath. It was observed that primordial follicles were located in the tunica albuginea layer and the fibroblast-like mesenchyme in the tunica albuginea of the corpus luteum formed after ovulation, thickens this layer by proliferation of cells and creates a suitable environment for the development of primordial follicles, also the tubules of the rete ovarii, which emerge during the formation of the genitourinary system in the embryological period, continue to exist in the ovaries and mesovarium in rats in the early and late reproductive period.
Conclusions: The presence of tubules of the rete ovarii in the ovary and mesovarium during the reproductive period may indicate that these structures are also important in folliculogenesis.

Keywords

Article Title [Persian]

تلاش برای درک فرآیند تخمدان موش صحرایی از طریق مقاطع بافت شناسی و مطالعات کشت سلولی

Authors [Persian]

  • مرات سرکانت اونال 1
  • سید مهدی طباطبایی 2
1 گروه بافت شناسی و جنین شناسی، دانشکده پزشکی، دانشگاه پاموکاله، دنیزلی، ترکیه
2 گروه بافت شناسی و جنین شناسی، دانشکده پزشکی، دانشگاه پاموکاله، دنیزلی، ترکیه- باشگاه پژوهشگران جوان و نخبگان، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد

Keywords [Persian]

  • ساختارهای اپوفورون
  • فولیکولوژنز
  • فولیکول گرااف
  • تخمدان
Iranian Journal of Veterinary Medicine

Articles in Press, Accepted Manuscript
Available Online from 24 January 2024

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