نوع مقاله : عوامل عفونی - بیماریها

نویسندگان

گروه میکروبشناسی، دانشکده دامپزشکی دانشگاه ارومیه، ارومیه، ایران

چکیده

زمینه مطالعه:  توجه فزاینده‌ای در مورد تأثیر حضور اکولای‌های مولد بتا-لاکتاماز وسیع الطیف جدا شده از دام‌ها بر روی بهداشت عمومی وجود دارد. هدف: هدف از این مطالعه بررسی حضور سه کلاس ژن ESBL در جدایه‌های اکولای بدست آمده از گوسفند و جوجه‌های گوشتی در سطح کشتار در منطقه ارومیه، ایران، می‌باشد. روش کار: در کل 111 جدایه اکولای از نمونه‌های مدفوع گوسفند (55=n) و جوجه‌های گوشتی (56=n) بدست آمد و حضور ژن‌های blaCTX-M،blaTEM و blaSHV توسط واکنش زنجیره‌ای پلیمراز (PCR) شناسایی شدند. نتایج: به طور کلی 32 مورد از این جدایه‌ها blaCTX-M، 16 مورد blaTEM، و 17 مورد blaCTX-M را به همراه blaTEM حمل می‌نمودند. هیچ‌کدام از جدایه‌ها برای ژن blaSHV مثبت نبودند. از 55 جدایه بدست آمده از گوسفند، 33 جدایه (60%) حاوی یک یا بیش از یک ژن کد کننده ESBL بودند؛ به طوری که 15 (2/27%)، 10 (2/18%) و 8 جدایه (5/14%) به ترتیب برای blaCTX-M، blaTEM و blaCTX-M + blaTEM مثبت بودند. در بین 56 جدایه بدست آمده از جوجه‌های گوشتی، 32 جدایه (1/57%) حداقل حامل یک ژن کد کننده ESBL بودند؛ به طوری که 17 (4/30%) و 6 جدایه (7/10%) به ترتیب برای ژن‌های blaCTX-M و blaTEM مثبت بودند و blaCTX-M + blaTEM در 9 جدایه (1/16%) شناسایی گردید. نتیجه‌گیری نهایی: این مطالعه نشان داد که مدفوع گوسفند و جوجه‌های گوشتی ممکن است مخزن اکولای‌های حامل ژن‌های ESBL، با غالبیت بتا-لاکتاماز نوع CTX-M، باشد. این موضوع ممکن است بهداشت عمومی را با خطر مواجه سازد که نیازمند ارزیابی و کنترل آتی است.

کلیدواژه‌ها


 
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